Evidence map›Paper›PMID 40108136›Full record

ArticleNature communications2025

Septin5 deletion enhances β-cell exocytosis by releasing microtubule-tethered insulin granules onto plasma membrane.

Li Xie, Fei Kang, Tairan Qin, Youhou Kang, Tao Liang, Huanli Xie, Carol D Froese, Hong Xie, Aaron Au, Christopher M Yip and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Li Xie *Department of Medicine, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada. li.xie@utoronto.ca.ORCID http://orcid.org/0000-0002-2727-0268
Fei Kang *Department of Medicine, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada. fei.kang@uhn.ca.ORCID http://orcid.org/0000-0001-7820-7173
Tairan QinDepartment of Medicine, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
Youhou KangDepartment of Medicine, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
Tao LiangDepartment of Medicine, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
Huanli XieDepartment of Medicine, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
Carol D FroeseThe program in Cell Biology, Department of Biochemistry, Hospital for Sick Children, University of Toronto, Toronto, ON, Canada.
Hong XieThe program in Cell Biology, Department of Biochemistry, Hospital for Sick Children, University of Toronto, Toronto, ON, Canada.
Aaron AuInstitute of Biomedical Engineering, University of Toronto, Toronto, ON, Canada.ORCID http://orcid.org/0000-0001-5717-048X
Christopher M YipInstitute of Biomedical Engineering, University of Toronto, Toronto, ON, Canada.ORCID http://orcid.org/0000-0003-4507-556X
William S TrimbleThe program in Cell Biology, Department of Biochemistry, Hospital for Sick Children, University of Toronto, Toronto, ON, Canada.ORCID http://orcid.org/0000-0001-9776-5111
Herbert Y GaisanoDepartment of Medicine, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada. herbert.gaisano@utoronto.ca.ORCID http://orcid.org/0000-0001-5213-9168

Funding

Gouvernement du Canada | Canadian Institutes of Health Research (Instituts de Recherche en Santé du Canada) CIHR-PJT-159741Gouvernement du Canada | Canadian Institutes of Health Research (Instituts de Recherche en Santé du Canada) MOP 86544
6 · The paper itself

Abstract

Septin5 interacts with SNARE proteins to regulate exocytosis in neurons, but its role in pancreatic β-cells is unknown. Here, we report that Septin5 is abundant in rodent and human β-cells, deletion of which dramatically enhances biphasic glucose-stimulated insulin secretion, including in type 2 diabetes (T2D). Super-resolution imaging shows that Septin5 is preferentially assembled in microtubule-plasma membrane contact sites in a microtubule-dependent manner, which provides discrete harbor for secretory granule anchoring. By decreasing the stability of the cortical microtubule meshwork, Septin5 depletion increases insulin granule dynamics and access to the plasma membrane. Analysis of spatiotemporal coupling of fusion events and localized Ca

Indexed as

Cell MembraneExocytosisInsulinInsulin-Secreting CellsMicrotubulesSecretory VesiclesSeptinsAnimalsCalciumDiabetes Mellitus, Type 2GlucoseHumansInsulin SecretionMaleMiceMice, Inbred C57BLCalciumGlucoseInsulinSeptins

Identifiers

PMID40108136
PMCPMC11923188

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.