ArticleMicrobial cell factories2025
Gluconobacter oxydans DSM 50049 - an efficient biocatalyst for oxidation of 5-formyl-2-furancarboxylic acid (FFCA) to 2,5-furandicarboxylic acid (FDCA).
Article in Microbial cell factories, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Microbial synthesis and degradation of bio-based biodegradable plastics.Nature reviews. Microbiology · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background2,5-Furandicarboxylic acid (FDCA) is a promising building block for biobased recyclable polymers and a platform for other potential biobased chemicals. The common route of its production is by oxidation of sugar-derived 5-hydroxymethylfurfural (HMF). Several reports on biocatalytic oxidation using whole microbial cells or enzymes have been reported, which offers potentially a greener alternative compared to the chemical process. HMF oxidases and aryl alcohol oxidases are the only enzymes able to catalyse the complete oxidation to FDCA, however at low concentrations and are subject to inhibition by the FFCA (5-formylfuran-2-carboxylic acid) intermediate. The present report presents a study on the oxidation of FFCA to FDCA using the obligately aerobic bacterium Gluconobacter oxydans and identification of the enzymes catalyzing the reaction.
resultsScreening of three different strains showed G. oxydans DSM 50049 to possess the highest FFCA oxidation efficiency. Optimal reaction conditions for obtaining 100% conversion of 10 g/L (71 mM) FFCA to FDCA at 100% reaction yield were at pH 5, 30 °C and using 200 mg wwt /mL cells harvested at mild-exponential phase. In a reaction run at a 1 L scale using a total of 15 g/L (107 mM) FFCA supplied in a fed-batch mode, FDCA was obtained at a yield of 90% in 8.5 h. The product was recovered at 82% overall yield and 99% purity using a simple recovery process. Screening of several oxidoreductase enzymes from the gene sequences identified in the bacterial genome revealed two proteins annotated as membrane-bound aldehyde dehydrogenase (MALDH) and coniferyl aldehyde dehydrogenase (CALDH) to be the enzymes catalyzing the oxidization of FFCA.
conclusionThe study shows G. oxydans DSM 50049 and its enzymes to be promising biocatalysts for use in the FDCA production process from biomass. The high reaction rate and yield motivate further studies on characterization of the identified enzymes exhibiting the FFCA oxidizing activity, which can be used to construct an enzyme cascade together e.g. with HMF oxidase or aryl alcohol oxidase for one-pot production of FDCA from 5-HMF.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.