ArticlePlant biotechnology journal2025
Efficient clonal seeds sorting for apomictic hybrid rice using a pollen-specific gene switch system.
Article in Plant biotechnology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- DNA Methylation Dynamics in Plant Abiotic Stress Response: Mechanisms, Memory, and Breeding Applications.Genes · 2026Review
- DNA methylation in plant heterosis: mechanisms and prospects.Functional & integrative genomics · 2025Review
- Research Advances in Multiple Embryos and Apomixis in Rice (International journal of molecular sciences · 2025Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Significant progress in apomictic hybrid rice development faces challenges like achieving high induction rates and seed-setting efficiencies, and distinguishing clonal from zygotic embryos. To address the challenge of selecting clonal seeds, we developed a dual-fluorescence labelling gene switch system using the recombinase Cre/LoxP + FRT. Initially, this system was tested in callus tissue under a constitutive promoter; then, we replaced the promoter with a pollen-specific one to develop the pollen-specific gene switch (PSGS) system. The effectiveness of PSGS in rice pollen was subsequently validated. After confirming its functionality, we co-transformed the PSGS vectors with apomixis vectors in hybrid rice Yongyou 2640 (YE) and Yongyou 4949 (YS) using Agrobacterium-mediated transformation. Finally, we identified 18 MiMe mutants carrying the PSGS; the progeny of 16 lines were all red fluorescence seeds (zygotic embryo). Surprisingly, line L47-4 and L151-1 yielded 418 (n = 418) and 218 (n = 1279) non-fluorescent seeds in the T
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.