Evidence map›Paper›PMID 40112813›Full record

ArticleNeuron2025

APOE genotype determines cell-type-specific pathological landscape of Alzheimer's disease.

Zonghua Li, Yuka A Martens, Yingxue Ren, Yunjung Jin, Hiroaki Sekiya, Sydney V Doss, Naomi Kouri, Monica Castanedes-Casey, Trace A Christensen, Lindsay B Miller Nevalainen and 20 more

Abstract read
In one paragraph

Article in Neuron, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed.

  1. Trial
  2. Review
  3. Article
  4. Article
  5. Review
  6. Review
  7. Article
  8. Article
  9. APOE*4 risk-modifying genes and drug targets in Alzheimer's disease through cell-type-specific genomic analyses.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2026
    Article
  10. Review
  11. Review
  12. Non-microglial downregulation ofbioRxiv : the preprint server for biology · 2026
    Article
  13. Genetic risk in Alzheimer's disease.NPJ systems biology and applications · 2026
    Review
  14. Article
  15. Article
  16. Review
  17. Review
  18. Article
  19. Article
  20. Emerging Role of Oligodendrocytes Malfunction in the Progression of Alzheimer's Disease.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

30 authors.

Zonghua LiDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Yuka A MartensDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Yingxue RenDepartment of Quantitative Health Sciences, Mayo Clinic, Jacksonville, FL 32224, USA.
Yunjung JinDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Hiroaki SekiyaDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Sydney V DossDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Naomi KouriDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Monica Castanedes-CaseyDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Trace A ChristensenMicroscopy and Cell Analysis Core, Mayo Clinic, Rochester, MN 55905, USA.
Lindsay B Miller NevalainenMicroscopy and Cell Analysis Core, Mayo Clinic, Rochester, MN 55905, USA.
Nanaka TakegamiDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Kai ChenDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Chia-Chen LiuDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Alexandra Soto-BeasleyDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Baayla D C BoonDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Sydney A LabuzanDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Tadafumi C IkezuDepartment of Quantitative Health Sciences, Mayo Clinic, Jacksonville, FL 32224, USA.
Yixing ChenDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Alexander D BartkowiakDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Gisela XhafkollariDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Allison M WetmoreDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
David A BennettRush Alzheimer's Disease Center, Rush University Medical Center, Chicago, IL 60612, USA.
Ross R ReichardDepartment of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.
Ronald C PetersenDepartment of Neurology, Mayo Clinic, Rochester, MN 55905, USA.
Takahisa KanekiyoDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Owen A RossDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Melissa E MurrayDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Dennis W DicksonDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Guojun BuDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA. Electronic address: gbu@ust.hk.
Na ZhaoDepartment of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA. Electronic address: zhao.na@mayo.edu.

Funding

SUPPLEMENT TO ALZHEIMERS DISEASE PATIENT REGISTRYU01AG006786 · NIA · MAYO CLINIC ROCHESTER · PI GRAFF-RADFORD, JONATHAN, JACK, CLIFFORD R. · 1986 to 2023
$49.6M
Project 4 (Genetic modifiers for APOE-associated Alzheimer's disease pathogenesis)U19AG069701 · NIA · MAYO CLINIC JACKSONVILLE · PI ALISON M GOATE · 2021 to 2026
$42.0M
Research Education ComponentP30AG062677 · NIA · MAYO CLINIC ROCHESTER · PI Pamela J McLean · 2019 to 2026
$33.5M
Rush Alzheimer's Disease Research CenterP30AG072975 · NIA · RUSH UNIVERSITY MEDICAL CENTER · PI Lisa L Barnes, Julie A. Schneider · 2021 to 2026
$24.7M
Utilization of proteomics and lipidomics to identify modifiers of LBDU54NS110435 · NINDS · MAYO CLINIC JACKSONVILLE · PI MCLEAN, PAMELA J · 2019 to 2023
$14.5M
Integrative translational discovery of vascular risk factors in aging and dementiaRF1AG051504 · NIA · MAYO CLINIC JACKSONVILLE · PI BU, GUOJUN, ERTEKIN-TANER, NILUFER · 2015 to 2019
$9.8M
TREM2-mediated microglial dynamic function in Alzheimer diseaseR01AG066395 · NIA · MAYO CLINIC JACKSONVILLE · PI ZHAO, NA · 2020 to 2024
$3.7M
Impact of ApoE2 on aging-related conditionsRF1AG057181 · NIA · MAYO CLINIC JACKSONVILLE · PI KANEKIYO, TAKAHISA · 2018 to 2018
$3.7M
ApoE isoform-specific therapy for Alzheimer diseaseRF1AG046205 · NIA · MAYO CLINIC JACKSONVILLE · PI ZHAO, NA · 2019 to 2019
$3.1M
ApoE pathway in cerebrovascular Aβ clearance in Alzheimer’s diseaseR37AG027924 · NIA · MAYO CLINIC JACKSONVILLE · PI BU, GUOJUN · 2017 to 2020
$2.4M
Elucidating the Pathomechanisms of APOE4 in Lewy Body DementiaR01AG087165 · NIA · MAYO CLINIC JACKSONVILLE · PI Owen A Ross, Na Zhao · 2024 to 2026
$2.3M
ApoE isoform-specific therapy for Alzheimer diseaseR01AG046205 · NIA · MAYO CLINIC JACKSONVILLE · PI BU, GUOJUN · 2013 to 2017
$1.6M
NIA NIH HHS P30 AG062677NIA NIH HHS P30 AG072975NIA NIH HHS R01 AG046205NIA NIH HHS R01 AG066395NIA NIH HHS R01 AG087165NIA NIH HHS R37 AG027924NIA NIH HHS RF1 AG046205NIA NIH HHS RF1 AG051504NIA NIH HHS RF1 AG057181NIA NIH HHS U01 AG006786NIA NIH HHS U19 AG069701NINDS NIH HHS U54 NS110435
6 · The paper itself

Abstract

The apolipoprotein E (APOE) gene is the strongest genetic risk modifier for Alzheimer's disease (AD), with the APOE4 allele increasing risk and APOE2 decreasing it compared with the common APOE3 allele. Using single-nucleus RNA sequencing of the temporal cortex from APOE2 carriers, APOE3 homozygotes, and APOE4 carriers, we found that AD-associated transcriptomic changes were highly APOE genotype dependent. Comparing AD with controls, APOE2 carriers showed upregulated synaptic and myelination-related pathways, preserving synapses and myelination at the protein level. Conversely, these pathways were downregulated in APOE3 homozygotes, resulting in reduced synaptic and myelination proteins. In APOE4 carriers, excitatory neurons displayed reduced synaptic pathways similar to APOE3, but oligodendrocytes showed upregulated myelination pathways like APOE2. However, their synaptic and myelination protein levels remained unchanged or increased. APOE4 carriers also showed increased pro-inflammatory signatures in microglia but reduced responses to amyloid-β pathology. These findings reveal APOE genotype-specific molecular alterations in AD across cell types.

Indexed as

Alzheimer DiseaseApolipoprotein E2Apolipoprotein E3Apolipoproteins EAgedAged, 80 and overApolipoprotein E4FemaleGenotypeHumansMaleMicrogliaNeuronsOligodendrogliaSynapsesTemporal LobeApolipoprotein E2Apolipoprotein E3Apolipoprotein E4Apolipoproteins EAlzheimer’s diseaseAPOEinflammationmicroglia activationmyelinationsingle-nucleus RNA sequencingsynaptic loss

Identifiers

PMID40112813
PMCPMC12333916

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.