ArticleTranslational andrology and urology2025
Application of decellularized tilapia skin in rabbit urethral reconstruction: an experimental study.
Article in Translational andrology and urology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: In urethral reconstruction, autologous tissue grafts are subject to various limitations. Decellularized tissue has attracted significant interest in tissue engineering (TE) and regenerative medicine due to great biocompatibility and bioactivity. We investigated the effect of decellularized tilapia skin (DTS) in a rabbit urethral defect model to explore its feasibility and effectiveness as a TE construction for urethral reconstruction. Methods: Fresh tilapia skins were decellularized and verified through its residual deoxyribonucleic acid (DNA) content measurement. The physical properties and degradation profile of DTS were characterized as well. Finally, we established a rabbit urethral defect model and evaluated the effect of DTS on urethral defect healing through pathological examination and immunohistochemistry (IHC) staining. Results: The residual DNA content in the DTS was 32.94 ng/mg. Electron microscopy showed that the DTS retained its three-dimensional structure. Conclusions: DTS could degrade gradually during urethral reconstruction and demonstrated its better biocompatibility in terms of
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