Evidence map›Paper›PMID 40117024›Full record

ArticleBioresources and bioprocessing2025

Comprehensive biochemical, molecular and structural characterization of subtilisin with fibrinolytic potential in bioprocessing.

Shreya S Shettar, Zabin K Bagewadi, Mohammed Alasmary, Basheerahmed Abdulaziz Mannasaheb, Ibrahim Ahmed Shaikh, Aejaz Abdullatif Khan

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioresources and bioprocessing, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Shreya S ShettarDepartment of Biotechnology, KLE Technological University, Vidyanagar, Hubballi, 580031, Karnataka, India.
Zabin K BagewadiDepartment of Biotechnology, KLE Technological University, Vidyanagar, Hubballi, 580031, Karnataka, India. zabin@kletech.ac.in.ORCID http://orcid.org/0000-0002-6650-7045
Mohammed AlasmaryDepartment of Medicine, College of Medicine, Najran University, 66462, Najran, Saudi Arabia.
Basheerahmed Abdulaziz MannasahebDepartment of Pharmacy Practice, College of Pharmacy, AlMaarefa University, P.O. Box 71666, 11597, Riyadh, Saudi Arabia.
Ibrahim Ahmed ShaikhDepartment of Pharmacology, College of Pharmacy, Najran University, 66462, Najran, Saudi Arabia.
Aejaz Abdullatif KhanDepartment of General Science, Ibn Sina National College for Medical Studies, 21418, Jeddah, Saudi Arabia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Enzyme deployment is proliferating extensively in industries owing to their environmentally friendly and easily degradable attributes. This article undertakes an exhaustive examination of wild subtilisin enzyme, covering purification, biochemical delineation, analytical techniques, and practical implementations. The purification methodology involved partial refinement, anionic exchange, and gel filtration chromatography, culminating in a purification factor of 3.406, corroborated by SDS-PAGE showcasing a molecular weight of ~ 42 kDa. Biochemical scrutiny unveiled the enzyme's response, with an optimal pH at 9 and temperature peak at 60 ℃. Various surfactants, metal ions, organic solvents and inhibitors exhibited notable efficacy. Substrate specificity and kinetics showcased the utmost specificity with N-Suc-F-A-A-F-pNA, registering K

Indexed as

ApplicationsBiochemical characterizationMolecular dockingMolecular dynamic simulationPurificationSubtilisin

Identifiers

PMID40117024
PMCPMC11928348

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.