Evidence mapPaperPMID 40123263Full record

ArticleMolecular nutrition & food research2025

Prenatal Intake of High Multivitamins or Folic Acid With or Without Choline Contributes to Gut Microbiota-Associated Dysregulation of Serotonin in Offspring.

Jianzhang Dong, Mali Al-Issa, Jenny S Feeney, Gia V Shelp, Elizabeth M Poole, Clara E Cho

Abstract read
In one paragraph

Article in Molecular nutrition & food research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jianzhang DongDepartment of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada.
Mali Al-IssaDepartment of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada.
Jenny S FeeneyDepartment of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada.
Gia V ShelpDepartment of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada.
Elizabeth M PooleDepartment of Family Relations and Applied Nutrition, University of Guelph, Guelph, Ontario, Canada.
Clara E ChoDepartment of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada.ORCID 0000-0002-9733-2516

Funding

Canadian Institutes of Health Research Canada Graduate Scholarship-MasterCanadian Institutes of Health Research Canada Research Chair Tier IINatural Sciences and Engineering Research Council of Canada Discovery GrantSummer Undergraduate Research AssistantshipUniversity of Guelph, College of Biological Science Team Building GrantUtah Agricultural Experiment Station Grant
6 · The paper itself

Abstract

The gut microbiota is amenable to early nutrition including micronutrients but intake above and below the recommendations commonly occur with unknown consequences. Serotonin (5-hydroxytryptamine [5-HT]) is a monoamine found centrally and peripherally with diverse functions such as food intake regulation via the hypothalamic 5-HT receptor 2C (5-HTR2C). This study determined the impact of prenatal micronutrients on the gut microbiota and serotonergic system in offspring. Pregnant Wistar rats were fed either recommended vitamins (RV), high vitamins (HV), high folic acid with recommended choline (HFRC), or high folic acid with no choline (HFNC). Offspring were fed a high-fat diet for 12 weeks postweaning. HV, HFRC, and HFNC males and females had lower hypothalamic 5-HTR2C protein expression compared to RV. Brain 5-HT concentrations were lower but colon 5-HT concentrations were higher in HV and HFNC males and females and HFRC males compared to RV. Refeeding response after 5-HTR2C agonist was negatively correlated with hypothalamic 5-HTR2C protein expression in males and with brain 5-HT concentrations in females. Random forest revealed top bacterial taxa, which Lactococcus, Ruminococcus, Bacteroides, and Oscillospira showed significant correlations with refeeding response and concentrations of brain and colon 5-HT. In conclusion, excess or imbalanced prenatal consumption of micronutrients leads to gut microbiota-associated disturbances in the serotonergic system in offspring.

Indexed as

CholineFolic AcidGastrointestinal MicrobiomeSerotoninVitaminsAnimalsBrainColonDiet, High-FatFemaleHypothalamusMalePregnancyPrenatal Exposure Delayed EffectsRatsRats, WistarCholineFolic AcidReceptor, Serotonin, 5-HT2CSerotoninVitaminsbraingut microbiotamicronutrientsserotoninserotonin receptor

Identifiers

PMID40123263
PMCPMC12050513

What Socratic holds

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.