Evidence map›Paper›PMID 40125696›Full record

ArticleCell proliferation2025

Accelerating Bone Healing With METTL3 Overexpressed Adipose-Derived Stem Cells in Osteoporotic Rats.

Hui Tang, Zhenzhen Chen, Lu Zeng, Yuping Xie, Daowen Luo, Shuanglin Peng, Fangzhi Lou, Tianli Wu, Jingang Xiao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell proliferation, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Effects of the Total Flavonoids fromCurrent molecular medicine · 2026
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Hui TangDepartment of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou, China.
Zhenzhen ChenDepartment of Oral Implantology, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou, China.
Lu ZengDepartment of Oral Implantology, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou, China.
Yuping XieDepartment of Oral Implantology, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou, China.
Daowen LuoLuzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou, China.
Shuanglin PengDepartment of Oral Implantology, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou, China.
Fangzhi LouDepartment of Oral Implantology, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou, China.
Tianli WuDepartment of Oral Implantology, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou, China.
Jingang XiaoDepartment of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou, China.ORCID https://orcid.org/0000-0003-1300-7191

Funding

General Program of Southwest Medical University 2023QN071National Natural Science Foundation of China 81870746National Natural Science Foundation of China 82370938Project of Stomatological Institute of Southwest Medical University 2021XJYJS01Science & Technology Department of Sichuan Province 2022YFS0634Science & Technology Department of Sichuan Province 2024NSFSC2087Scientific Research Foundation of The Affiliated Stomatological Hospital of Southwest Medical University 2022Y01Special Project for Local Science and Technology Development Guided by the Central Government of Sichuan Province 2022ZYD0082Youth Foundation of Southwest Medical University 2021ZKQN036Youth Science Climbing Program of The Affiliated Stomatological Hospital of Southwest Medical University 2022KQ01
6 · The paper itself

Abstract

The treatment of postmenopausal osteoporosis (OP) presents a multifaceted challenge. Nonetheless, emerging research indicates a significant association between the N6-methyladenosine (m6A) methylase METTL3 and osteogenesis in OP. To investigate Mettl3's impact on osteogenic potential and the underlying molecular mechanisms, an OP rat model was established via ovariectomy (OVX). Osteoporotic adipose-derived stem cells (OP-ASCs) were then isolated. Results indicated a significant downregulation of Mettl3 expression in OP-ASCs. Subsequently, OP-ASCs were transfected with overexpressed Mettl3 lentivirus and treated for Dickkopf-related protein-1 (DKK1). Overexpression of the Mettl3 gene led to increased levels of osteogenic factors. DKK1 attenuated osteoblastic differentiation capacity in the Mettl3 overexpression group by inhibiting the Wnt signalling pathway. Consistent results were observed in vivo experiments. In conclusion, overexpression of Mettl3 promotes osteogenesis in OP-ASCs by activating the Wnt/β-catenin pathway.

Indexed as

Adipose TissueMethyltransferasesOsteoporosisOsteoporosis, PostmenopausalStem CellsAnimalsCell DifferentiationFemaleIntercellular Signaling Peptides and ProteinsOsteoblastsOsteogenesisOvariectomyRatsRats, Sprague-DawleyWnt Signaling PathwayDkk1 protein, ratIntercellular Signaling Peptides and ProteinsMethyltransferasesMETTL3N6‐methyladenosineOP‐ASCsosteoporosisWnt signalling pathway

Identifiers

PMID40125696
PMCPMC12414637

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.