Evidence map›Paper›PMID 40126708›Full record

ArticleJournal of molecular histology2025

In vivo and in silico analysis of anti inflammatory, antipyretic and analgesic activity of methanolic extract of Nigella sativa.

Nureen Zahra, Saher Fatima, Anum Nazir, Syeda Yumna Farrukh, Ayesha Anwer, Abid Sarwar, Tariq Aziz, Fahad Al Asmari, Aziza Mahdy Nahari, Rewa S Jalal and 2 more

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of molecular histology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Analysis of Pharmacological Properties ofLife (Basel, Switzerland) · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Nureen ZahraInstitute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Pakistan. naureen.zahra@imbb.uol.edu.pk.
Saher FatimaInstitute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Pakistan.
Anum NazirInstitute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Pakistan.
Syeda Yumna FarrukhInstitute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Pakistan.
Ayesha AnwerInstitute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Pakistan.
Abid SarwarFood and Biotechnology Research Lab, PCSIR Complex Lahore, Lahore 54590 Punjab, Pakistan.
Tariq AzizLaboratory of Animal Health Hygiene and Quality, Department of Agriculture, University of Ioannina, Arta, Greece. iwockd@gmail.com.
Fahad Al AsmariDepartment of Food and Nutrition Sciences, College of Agricultural and Food Sciences, King Faisal University, Al Ahsa, Saudi Arabia.
Aziza Mahdy NahariDepartment of Biological Sciences, College of Sciences, University of Jeddah, 21493, Jeddah, Saudi Arabia.
Rewa S JalalDepartment of Biological Sciences, College of Sciences, University of Jeddah, 21493, Jeddah, Saudi Arabia.
Fakhria A Al-JoufiDepartment of Pharmacology, College of Pharmacy, Jouf University, 72341, Aljouf, Saudi Arabia.
Maher S AlwethaynaniDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Alquwayiyah, Riyadh, Saudi Arabia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nigella Sativa (N. sativa) belongs to the family of Ranunculaceae and is an annual herb which is also known as black cumin or black seed. Since ancient times N. sativa has been used because of its widespread therapeutic properties which has been proven effective in respiratory, cardiovascular, gastrointestinal, inflammatory conditions and in inflammation. This study investigates the antiinflammatory, antipyretic, and analgesic properties of Nigella sativa (N. sativa) methanol extract using albino rats (n = 36). Diclofenac and paracetamol were used as standard medications in the trial, and the four concentrations of the methanolic extract (250, 500, 1000, and 2000 mg/kg) were tested for their effects on inflammation, pain, and fever. The methanolic extract of N. sativa seeds showed significant inhibition of fever (96%), inflammation (89%), and pain (85%). In addition, bioactive compounds in the seeds, including thymol, p-cymene, and limonene, were examined through in-silico studies. Ligand molecules and proteins associated with anti-inflammatory, analgesic, and antipyretic effects were 1DFN, 1A06, and 3LN0, respectively. The molecular docking results indicated significant binding interactions, with effective binding energy values corresponding to analgesic, antipyretic, and anti-inflammatory properties. Both in-silico and in-vivo results demonstrate the efficacy of N. sativa methanol extract in alleviating pain, inflammation, and fever.

Indexed as

AnalgesicsAnti-Inflammatory AgentsAntipyreticsNigella sativaPlant ExtractsAnimalsComputer SimulationFeverInflammationMaleMethanolMolecular Docking SimulationPainRatsAnalgesicsAnti-Inflammatory AgentsAntipyreticsMethanolPlant ExtractsDMSOIn-silicoIn vivoN. sativaPhytochemical compoundsThymoquinone

Identifiers

PMID40126708

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.