Evidence map›Paper›PMID 40136980›Full record

ReviewBiosensors2025

Nanobody-Based Immunoassays for the Detection of Food Hazards-A Review.

Wenkai Li, Zhihao Xu, Qiyi He, Junkang Pan, Yijia Zhang, El-Sayed A El-Sheikh, Bruce D Hammock, Dongyang Li

Abstract readReview
In one paragraph

Review in Biosensors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Advances in Immunological Methods for the Detection ofSensors (Basel, Switzerland) · 2026
    Review
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Wenkai LiCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
Zhihao XuCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
Qiyi HeCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
Junkang PanCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
Yijia ZhangCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
El-Sayed A El-SheikhFaculty of Agriculture, Zagazig University, Zagazig 44511, Sharkia, Egypt.ORCID 0000-0003-0397-6650
Bruce D HammockDepartment of Entomology and Nematology and UCD Comprehensive Cancer Center, University of California Davis, Davis, CA 95616, USA.ORCID 0000-0003-1408-8317
Dongyang LiCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.

Funding

Workshop on Environmental Technology Transfer and EntrepreneurshipP42ES004699 · NIEHS · UNIVERSITY OF CALIFORNIA DAVIS · PI YOUNG, THOMAS MICHAEL · 1987 to 2021
$50.1M
NIEHS NIH HHS P42 ES004699
6 · The paper itself

Abstract

Food safety remains a significant global challenge that affects human health. Various hazards, including microbiological and chemical threats, can compromise food safety throughout the supply chain. To address food safety issues and ensure public health, it is necessary to adopt rapid, accurate, and highly specific detection methods. Immunoassays are considered to be an effective method for the detection of highly sensitive biochemical indicators and provide an efficient platform for the identification of food hazards. In immunoassays, antibodies function as the primary recognition elements. Nanobodies have significant potential as valuable biomolecules in diagnostic applications. Their distinctive physicochemical and structural characteristics make them excellent candidates for the development of reliable diagnostic assays, and as promising alternatives to monoclonal and polyclonal antibodies. Herein, we summarize a comprehensive overview of the status and prospects of nanobody-based immunoassays in ensuring food safety. First, we begin with a historical perspective on the development of nanobodies and their unique characteristics. Subsequently, we explore the definitions and boundaries of immunoassays and immunosensors, before discussing the potential applications of nanobody-based immunoassays in food safety testing that have emerged over the past five years, and follow the different immunoassays, highlighting their advantages over traditional detection methods. Finally, the directions and challenges of nanobody-based immunoassays in food safety are discussed. Due to their remarkable sensitivity, specificity and versatility, nanobody-based immunoassays hold great promise in revolutionizing food safety testing and ensuring public health and well-being.

Indexed as

Biosensing TechniquesFood SafetySingle-Domain AntibodiesFood ContaminationHumansImmunoassaySingle-Domain Antibodiesfood hazardsfood safetyimmunoassaynanobody

Identifiers

PMID40136980
PMCPMC11939871

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.