Evidence map›Paper›PMID 40138633›Full record

ArticleEnvironmental health perspectives2025

Neurological Outcomes of Joint Exposure to Polystyrene Micro/Nanospheres and Silver Nanoparticles in Zebrafish.

Jie Song, Qian Pu, Chen Chen, Xingcheng Liu, Xinlei Zhang, Zejun Wang, Jin Yan, Xuedong Wang, Huili Wang, Qiuhui Qian

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Environmental health perspectives, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Review
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jie SongNational and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, China.
Qian PuNational and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, China.
Chen ChenNational and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, China.
Xingcheng LiuNational and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, China.
Xinlei ZhangNational and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, China.
Zejun WangNational and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, China.
Jin YanNational and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, China.
Xuedong WangNational and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, China.
Huili WangNational and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, China.
Qiuhui QianNational and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMicro/nanoplastics and silver nanoparticles (AgNPs) are emerging environmental contaminants widely detected in aquatic environments. However, previous research has primarily focused on the interactions between micro/nanoplastics and organic substances or heavy metals, whereas the interactions and combined toxic effects of micro/nanoplastics with AgNPs remain unclear.

objectiveOur study aimed to investigate the effects and mechanisms of coexposure to AgNPs and polystyrene micro/nanospheres (PS M/NPs) on the nervous system, comparing the toxicity of AgNPs alone and in combination with PS M/NPs in larval zebrafish.

methodsWe investigated the dynamics of AgNPs' (

resultsAdsorption experiments showed PS M/NPs could stably adsorb AgNPs, with higher adsorption in smaller particles. Zebrafish larvae exposed to combined PS M/NPs and AgNPs demonstrated neurodevelopmental abnormalities, including developmental malformations, lower levels of locomotor activity, delayed response, and abnormal neuronal development. In addition, exposed zebrafish also exhibited disrupted neurodevelopmental markers, including vascular and apoptotic indicators, and oxidative stress and neuroimmune responses. Quantitative real-time polymerase chain reaction analysis showed differences in gene expression within neurotoxic pathways in PS M/NPs and AgNPs-exposed zebrafish, focusing on key genes in immunity, apoptosis, vascular, and neural development. Furthermore, these neurotoxic effects induced by combined exposure were alleviated following the introduction of the neuroinflammation inhibitor curcumin. DISCUSSION: Our findings demonstrate that polystyrene nanospheres (PSNPs) intensified AgNPs-induced neurotoxicity in larval zebrafish, whereas polystyrene microspheres (PSMPs) had a lesser effect, indicating distinct gene regulation roles when combined with AgNPs. These findings enhance the assessment of environmental risks in settings with coexisting nanomaterials and microplastics, offering important insights for evaluating combined exposure risks. https://doi.org/10.1289/EHP14873.

Indexed as

Metal NanoparticlesNanospheresPolystyrenesSilverWater Pollutants, ChemicalZebrafishAnimalsLarvaMicroplasticsOxidative StressMicroplasticsPolystyrenesSilverWater Pollutants, Chemical

Identifiers

PMID40138633
PMCPMC12068508

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.