Evidence map›Paper›PMID 40139733›Full record

ArticleNano letters2025

From Plants to Plants: Plant-Derived Biological Polymers as Sustainable and Safe Nanocarriers for Direct Delivery of DNA to Plant Cells.

Kari Vinzant, Mohammad Rashid, Delaney E Clouse, Pratyusha Ghosh, Mohiuddin Quadir, Virginia A Davis, Mariya V Khodakovskaya

Abstract read
In one paragraph

Article in Nano letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kari VinzantUniversity of Arkansas at Little Rock, Little Rock, Arkansas 72204, United States.
Mohammad RashidUniversity of Arkansas at Little Rock, Little Rock, Arkansas 72204, United States.
Delaney E ClouseAuburn University, Auburn, Alabama 36849, United States.
Pratyusha GhoshNorth Dakota State University, Fargo, North Dakota 58108, United States.
Mohiuddin QuadirNorth Dakota State University, Fargo, North Dakota 58108, United States.ORCID 0000-0003-2811-773X
Virginia A DavisAuburn University, Auburn, Alabama 36849, United States.ORCID 0000-0003-3126-3893
Mariya V KhodakovskayaUniversity of Arkansas at Little Rock, Little Rock, Arkansas 72204, United States.ORCID 0000-0001-6398-4105

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sustainable plant-derived biopolymers cellulose nanocrystals (CNC) and Zein protein were used to deliver plasmid DNA with a reporter GFP gene (pDNA) to plant cells. CNC and Zein were modified with the cationic agent 2,3-epoxypropyltrimethylammonium chloride (EPTMAC) to electrostatically bind the biopolymers to negatively charged pDNA. Established pDNA-CNC and pDNA-Zeins conjugates were delivered to tobacco cells by leaf injection and vacuum infiltration of tobacco leaves and seedlings. Both methods effectively provided transient GFP expression in exposed plant cells that was visualized by confocal microscopy and confirmed by qRT-PCR (GFP gene expression) and Western blot (GFP protein expression). Our findings support the idea that nanopolymers derived from agricultural waste residues can successfully be used to advance plant transformation and gene editing. Delivering genetic material using biocompatible, plant-based nanopolymers in large-scale vacuum infiltration of plant tissues reduces existing limitations of plant transformation and increases the speed of the transformation process.

Indexed as

CelluloseDNAGene Transfer TechniquesNanoparticlesNicotianaZeinGreen Fluorescent ProteinsPlant LeavesPlasmidsCelluloseDNAGreen Fluorescent ProteinsZeincellulose nanocrystalscyclic agriculturenanocarriersnatural polymersplant genetic modificationplant transformationpolymeric nanoparticlessustainabilityzein

Identifiers

PMID40139733
PMCPMC11987027

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.