ArticleViruses2025
Evaluation of Saliva Collection and DNA Extraction Methods for Practical Application of Salivary Human Herpesvirus 6 and 7 Assays.
Article in Viruses, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- A pilot study: Salivary human herpesvirus-6 and human herpesvirus-7 responses to different types of acute exercise in healthy young men.Physiological reports · 2025Trial
- Observational
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Salivary human herpesvirus 6 and/or 7 (HHV-6/7) have recently attracted attention as microbiological markers of physiological fatigue in laborers and athletes. However, the accuracy and efficiency of the HHV-6/7 assays can be improved for practical application. We conducted three experiments to identify suitable saliva collection and DNA extraction methods for practical salivary HHV-6/7 assays. The main experiment compared the data, including template DNA or HHV-6/7 concentrations, among three saliva collection methods (cotton, synthetic, and no swabs) and two DNA extraction methods (magnetic bead-based and silica column-based). It showed that using swabs had adverse effects: lower template DNA concentration, lower HHV-6/7 detection rates, higher coefficient of variation values, and lower concentrations. Moreover, magnetic bead-based methods resulted in higher HHV-6/7 detection rates and lower coefficient of variation values. Sub-experiment 1 examined practical saliva collection methods and demonstrated that the stimulated spitting method could collect saliva in a shorter time with lower subjective stress than the unstimulated spitting and stimulated swabbing methods. Sub-experiment 2 investigated diurnal variation in salivary HHV-6/7 levels but did not show diurnal variation. These findings suggest that (1) the combination of stimulated spitting saliva collection and magnetic bead-based DNA extraction is most suitable for practical salivary HHV-6/7 assays, and (2) saliva collection can be conducted whenever needed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.