Evidence map›Paper›PMID 40148443›Full record

ArticleScientific reports2025

Green synthesis, characterization, and antimicrobial activity of silver nanoparticles from water-soluble fractions of Brazilian Kefir.

Lucas Matos Martins Bernardes, Serena Mares Malta, Ana Carolina Costa Santos, Rafael Alves da Silva, Tamiris Sabrina Rodrigues, Murillo Néia Thomaz da Silva, Vinicius Prado Bittar, Ana Luiza Silva Borges, Allisson Benatti Justino, Arlene Bispo Dos Santos Nossol and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Lucas Matos Martins BernardesGenetics Laboratory, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil. lucasmatosbernardes@hotmail.com.
Serena Mares MaltaGenetics Laboratory, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil.
Ana Carolina Costa SantosGenetics Laboratory, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil.
Rafael Alves da SilvaGenetics Laboratory, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil.
Tamiris Sabrina RodriguesGenetics Laboratory, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil.
Murillo Néia Thomaz da SilvaLaboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, Brazil.
Vinicius Prado BittarLaboratory of Biochemistry and Molecular Biology, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, Brazil.
Ana Luiza Silva BorgesLaboratory of Biochemistry and Molecular Biology, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, Brazil.
Allisson Benatti JustinoLaboratory of Biochemistry and Molecular Biology, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, Brazil.
Arlene Bispo Dos Santos NossolLaboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, Brazil.
Mário Machado MartinsLaboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, Brazil.
Foued Salmen EspíndolaLaboratory of Biochemistry and Molecular Biology, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, Brazil.
Ana Paula Mendes-SilvaDepartment of Psychiatry, College of Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
Carlos Ueira-VieiraGenetics Laboratory, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil. ueira@ufu.br.

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico 403193/2022-2Fundação de Amparo à Pesquisa do Estado de Minas Gerais APQ-00269-22Fundação de Amparo à Pesquisa do Estado de Minas Gerais APQ-02766-17
6 · The paper itself

Abstract

Nanotechnology offers promising new avenues for combating drug-resistant pathogens. Given its antioxidant capacity, the water-soluble fraction of Brazilian kefir was hypothesized to serve as an effective reducing agent for the green synthesis of silver nanoparticles (AgNPs). It was further hypothesized that both the entire fraction (WSF) and the < 10 kDa fraction AgNPs would augment the therapeutic effects of kefir, particularly its antimicrobial activity. The successful synthesis was confirmed through the use of UV-Visible spectroscopy and Fourier-transform infrared analyses. WSF-AgNPs demonstrated potent antimicrobial activity, with minimum inhibitory concentrations of 25 µg/mL against A. baumannii (p < 0.0001) and 50 µg/mL against K. pneumoniae (p < 0.0001). Although no toxicity was observed in long-term tests on adult Drosophila melanogaster, AgNPs intake impaired larvae development. Oxidative stress analysis showed mild oxidative imbalance on advanced oxidation protein products (AOPP), sulfhydryl, and reduced glutathione (GSH) contents, with no alterations observed in reactive oxygen species (ROS) quantities, ferric reducing antioxidant power (FRAP), and catalase (CAT) activity. These findings suggest that kefir-derived AgNPs may have potential for combating drug-resistant infections. Future studies should focus on enhancing specificity through compound conjugation and investigating broader applications, including disinfectants, wound healing, and antibiotic development.

Indexed as

Anti-Bacterial AgentsAnti-Infective AgentsGreen Chemistry TechnologyKefirMetal NanoparticlesSilverAnimalsAntioxidantsBrazilDrosophila melanogasterMicrobial Sensitivity TestsOxidative StressSolubilityWaterAnti-Bacterial AgentsAnti-Infective AgentsAntioxidantsSilverWaterAnti-bacterial agentsKefirNanomedicineSilver nanoparticles

Identifiers

PMID40148443
PMCPMC11950517

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.