A novel strategy for rapid, portable, and sensitive detection of Clostridium perfringens cpb2 gene using smartphone-based electrochemical DNA biosensor based on screen-printed electrodes modified with nanocomposite.

Qianyue Wu, Beiying Yang, Yutang Wang, Yanyan Xiong, Jinhua Zhang, Yangping Wen, Andreja Rajkovic

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Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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7 authors.

Qianyue WuInstitute of Animal Disease Prevent and Control, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China.

Beiying YangInstitute of Animal Disease Prevent and Control, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China.

Yutang WangInstitute of Functional Materials and Agricultural Applied Chemistry, College of Chemistry and Materials, Jiangxi Agricultural University, Nanchang, 330045, China.

Yanyan XiongInstitute of Animal Disease Prevent and Control, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China.

Jinhua ZhangInstitute of Animal Disease Prevent and Control, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China. zhangjh1122@jxau.edu.cn.

Yangping WenInstitute of Functional Materials and Agricultural Applied Chemistry, College of Chemistry and Materials, Jiangxi Agricultural University, Nanchang, 330045, China. wenyangping1980@jxau.edu.cn.

Andreja RajkovicLaboratory of Food Microbiology and Food Preservation, Department of Food Technology, Safety and Health, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

An accurate, sensitive, low-cost, portable, and easy-to-use method for the quantitative detection of Clostridium perfringens (Cp), a zoonotic pathogen widely found in nature and capable of spreading through contaminated food or environments, is essential for epidemiology, prevention, and diagnostics. Here, we have designed a smartphone-based electrochemical DNA biosensor, which utilizes carboxyl-functionalized multi-walled carbon nanotubes (COOH-MWCNTs) as the substrate, L-cysteine (L-Cys) as the linker, and a synergistic modification of the bioelectrode with gold nanoparticles (AuNPs) and polyamidoamine dendrimers (PAMAM). This design enables highly sensitive, low-cost, label-free, and portable detection of the cpb2 gene in Cp. The sensor not only enhances detection performance but also improves convenience and practicality. Differential pulse voltammetry (DPV) was used to monitor the electrochemical signal response to changes in target DNA concentration, enabling the tracking of the DNA hybridization process. Under optimized conditions, the biosensor exhibited a linear detection range from 10⁻

Indexed as

Bacterial ToxinsBiosensing TechniquesClostridium perfringensDNA, BacterialElectrochemical TechniquesNanocompositesSmartphoneElectrodesGoldLimit of DetectionMetal NanoparticlesNanotubes, CarbonNucleic Acid HybridizationBacterial ToxinsDNA, BacterialGoldNanotubes, CarbonClostridium perfringens cpb2 geneDifferential pulse voltammetryElectrochemical gene biosensorPortable detectionVeterinary medicine

Identifiers

PMID40148726

DOI10.1016/j.foodchem.2024.141228

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.