Evidence map›Paper›PMID 40149974›Full record

ArticleBiomolecules2025

Efficient Fabrication of Human Corneal Stromal Cell Spheroids and Promoting Cell Stemness Based on 3D-Printed Derived PDMS Microwell Platform.

Yuexi Chen, Jianing Gu, Zekai Cui, Xihao Sun, Yuqin Liang, Chunwen Duan, Xiaoxue Li, Zhanyu Su, Bo Zhang, Jiansu Chen and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecules, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yuexi ChenThe First Clinical Medical College, Jinan University, Guangzhou 510632, China.
Jianing GuAier Academy of Ophthalmology, Central South University, Changsha 410015, China.
Zekai CuiAier Academy of Ophthalmology, Central South University, Changsha 410015, China.
Xihao SunAier Academy of Ophthalmology, Central South University, Changsha 410015, China.
Yuqin LiangAier Academy of Ophthalmology, Central South University, Changsha 410015, China.
Chunwen DuanAier Academy of Ophthalmology, Central South University, Changsha 410015, China.ORCID 0009-0000-9942-0918
Xiaoxue LiAier Academy of Ophthalmology, Central South University, Changsha 410015, China.
Zhanyu SuAier Academy of Ophthalmology, Central South University, Changsha 410015, China.
Bo ZhangGuangzhou Aier Eye Institute, Guangzhou 510071, China.
Jiansu ChenThe First Clinical Medical College, Jinan University, Guangzhou 510632, China.
Zheng WangThe First Clinical Medical College, Jinan University, Guangzhou 510632, China.

Funding

Aier Eye Group Research Foundation AMF2409D03the Natural Science Foundation of Hunan Province 2024JJ9011the Natural Science Foundation of Hunan Province 2024JJ9038the Xiangjiang Public Welfare Foundation KY24006
6 · The paper itself

Abstract

Spherical culture could promote the plasticity and stemness of human corneal stromal cells (hCSCs). Here, we introduce a novel three-dimensional (3D) cell culture system based on a polydimethylsiloxane (PDMS) microwell platform composed of many V-bottom microcavities to generate human corneal stromal cell spheroids and promote cell stemness. We isolated hCSCs from SMILE-derived lenticules and maintained their physiological phenotype by culturing them in a medium supplemented with human corneal stromal extract (hCSE). Utilizing a PDMS microwell platform fabricated through 3D printing technology, we successfully generated 3D corneal stromal cell spheroids (3D-CSC) with uniform size and stable structure, exhibiting increased expression of pluripotency factors, including OCT4, NANOG, SOX2, KLF4, and PAX6. Furthermore, the iPS supernatant of E8-conditioned medium (E8-CM) significantly enhanced the stemness properties of these cells. RNA sequencing and proteomics analyses revealed that 3D-CSCs exhibited superior proliferation, differentiation, cell adhesion, migration, and neurogenesis compared to traditional monolayer cultures, underscoring the role of biophysical cues in promoting hCSCs stemness. In summary, this study presents an effective 3D cell culture platform that mimics the in vivo microenvironment, facilitating the enhancement of stemness properties and providing valuable insights into corneal tissue engineering and regenerative medicine, particularly for treating corneal opacities and diseases.

Indexed as

Corneal StromaDimethylpolysiloxanesPrinting, Three-DimensionalSpheroids, CellularCell DifferentiationCell ProliferationCells, CulturedHumansKruppel-Like Factor 4baysilonDimethylpolysiloxanesKLF4 protein, humanKruppel-Like Factor 4human corneal stromal cellsPDMSpluripotencySMILEstem cellsstemnessthree-dimensional spheroid culture

Identifiers

PMID40149974
PMCPMC11940411

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.