Evidence map›Paper›PMID 40149985›Full record

ArticleBiomolecules2025

Structural and Metabolic Changes in Pregnant Rat Uterine and Adipose Tissue Induced by a High-Fat High-Sugar Diet.

Dina Šišljagić, Senka Blažetić, Milorad Zjalić, Irena Labak, Vedrana Ivić, Kálmán Ferenc Szűcs, Róbert Gáspár, Eszter Ducza, Sandor G Vari, Andrijana Muller and 1 more

Abstract read
In one paragraph

Article in Biomolecules, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Dina ŠišljagićClinic for Obstetrics and Gynecology, Clinical Hospital Center Osijek, 31000 Osijek, Croatia.
Senka BlažetićDepartment of Biology, Josip Juraj Strossmayer University of Osijek, 31000 Osijek, Croatia.
Milorad ZjalićDepartment for Medical Biology and Genetics, Faculty of Medicine Osijek, Josip Juraj Strossmayer University of Osijek, 31000 Osijek, Croatia.
Irena LabakDepartment of Biology, Josip Juraj Strossmayer University of Osijek, 31000 Osijek, Croatia.ORCID 0000-0001-9394-5131
Vedrana IvićDepartment for Medical Biology and Genetics, Faculty of Medicine Osijek, Josip Juraj Strossmayer University of Osijek, 31000 Osijek, Croatia.ORCID 0000-0002-8185-1960
Kálmán Ferenc SzűcsHungary BiopharmacyDepartment of Pharmacology and Pharmacotherapy, Albert-Szent-Györgyi Medical School, University of Szeged, 6720 Szeged, Hungary.ORCID 0000-0002-5878-4691
Róbert GáspárHungary BiopharmacyDepartment of Pharmacology and Pharmacotherapy, Albert-Szent-Györgyi Medical School, University of Szeged, 6720 Szeged, Hungary.ORCID 0000-0002-1571-7579
Eszter DuczaDepartment of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, 6720 Szeged, Hungary.
Sandor G VariInternational Research and Innovation in Medicine Program, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.ORCID 0000-0003-2962-2017
Andrijana MullerClinic for Obstetrics and Gynecology, Clinical Hospital Center Osijek, 31000 Osijek, Croatia.ORCID 0000-0003-3245-8318
Marija HefferDepartment for Medical Biology and Genetics, Faculty of Medicine Osijek, Josip Juraj Strossmayer University of Osijek, 31000 Osijek, Croatia.ORCID 0000-0001-6770-7359

Funding

Croatian Science Foundation IP-2014-2324Department of biology, Josip Juraj Strossmayer University of Osijek OZB - ZP 2022- BlažetićRECOOP HST Association, Cedars-Sinai Medical Center, Los Angeles, CA, USA 2018-2020 #012University of Osijek ZUP-2018 - Blažetić
6 · The paper itself

Abstract

Pregnancy presents specific metabolic demands, and disruption caused by a high-fat high-sugar diet (HFHSD) have been associated with significant complications, including maternal health risk, fetal developmental issues, and infertility. Obesity-related changes in the uterine tissues may contribute to these challenges. This study analyzed structural changes in the uterus and adipose tissue of pregnant rats on gestation day 22 fed an HFHSD using various staining techniques. Hematoxylin and eosin staining showed morphological changes in the adipose tissue and the uterine structure, including the lumen size and the thickness of the myometrium, endometrium, and perimetrium. The amount of collagen in the uterus was determined by PicroSirius red staining, while PAS-D staining was used to observe glycogen content. Key protein expressions, such as insulin and leptin receptors and UCP1 and UCP3, were analyzed by immunohistochemistry. The HFHSD promoted hypertrophy of visceral and gonadal adipocytes, suggesting metabolic alterations. By the end of pregnancy, a significant reduction in uterine lumen size was observed. Additionally, a decrease in insulin and higher leptin receptor expressions in the myometrium indicated significant physiological alteration. These findings offer insight into how an HFHSD affects uterine structure and function during late pregnancy but should be interpreted within the physiological context of gestation-related metabolic changes. Further research is needed to understand the functional consequences of these alterations on reproductive and metabolic health.

Indexed as

Adipose TissueDiet, High-FatUterusAnimalsFemaleInsulinPregnancyRatsReceptors, LeptinUncoupling Protein 1InsulinReceptors, LeptinUncoupling Protein 1endometriuminsulin receptorleptin receptormyometriumUCP proteins

Identifiers

PMID40149985
PMCPMC11940457

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.