Evidence map›Paper›PMID 40150730›Full record

ArticleBioengineering (Basel, Switzerland)2025

Extending the Three-Dimensional Culture of Adipocytes Through Surface Coatings.

Sheetal Chowdhury, Komal Beeton, Zacchaeus Wallace, Maggie Moore, Gene L Bidwell, Amol V Janorkar

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioengineering (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sheetal ChowdhuryDepartment of Biomedical Materials Science, University of Mississippi Medical Center, 2500 N State Street, Jackson, MS 39216, USA.ORCID 0000-0002-0739-4183
Komal BeetonDepartment of Cell and Molecular Biology, University of Mississippi Medical Center, 2500 N State Street, Jackson, MS 39216, USA.ORCID 0009-0003-0030-6886
Zacchaeus WallaceDepartment of Biomedical Materials Science, University of Mississippi Medical Center, 2500 N State Street, Jackson, MS 39216, USA.ORCID 0009-0008-5369-900X
Maggie MooreDepartment of Biomedical Materials Science, University of Mississippi Medical Center, 2500 N State Street, Jackson, MS 39216, USA.
Gene L BidwellDepartment of Neurology, University of Mississippi Medical Center, 2500 N State Street, Jackson, MS 39216, USA.ORCID 0000-0001-9104-150X
Amol V JanorkarDepartment of Biomedical Materials Science, University of Mississippi Medical Center, 2500 N State Street, Jackson, MS 39216, USA.

Funding

3D Spheroid Model of Adipose PathophysiologyR01EB020006 · NIBIB · UNIVERSITY OF MISSISSIPPI MED CTR · PI JANORKAR, AMOL VIJAY · 2016 to 2019
$1.4M
American Heart Association 24PRE1194432NIBIB NIH HHS R01 EB020006NIH HHS 1R01EB020006-05A1
6 · The paper itself

Abstract

To mimic the important features of progressing adiposity, in vitro adipose cell culture models must allow gradual intracellular fat accumulation in the three-dimensional (3D) arrangement of adipose-derived stem cells (ASCs) over a long-term culture period. Previously, elastin-like polypeptide (ELP) and polyethyleneimine (PEI) have been used to culture human adipose-derived stem cells (hASCs) as 3D spheroids and to differentiate them to adipocytes over a relatively long culture period of up to 5 weeks. In this study, to further enhance the spheroid adhesion properties, ELP was fused with Arginine-Glycine-Aspartic Acid (RGD) residues, known for their role as cell-attachment sites. This study aimed to assess whether the addition of RGD to the C-or N-terminus of ELP would impact the spheroid-forming ability of ELP-PEI coatings. ELP-RGD conjugates were produced using genetically modified

Indexed as

elastin-like polypeptideRGDspheroid retentionthree-dimensional spheroids

Identifiers

PMID40150730
PMCPMC11939550

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.