Toxicity Profiling of a Polyherbal formulation for hepatic health: acute and subacute evaluation.

All-natural products must be examined for any potential risks before being placed on the market. In this work, a polyherbal formulation with hepatoprotective properties was evaluated for acute, subacute, and subchronic toxicity. To examine the polyherbal syrup's toxicological profile in Wistar Albino rats. In compliance with OECD recommendations 423 and 407, acute and repeated dosage toxicity tests were carried out. A single oral dose of 2000 mg/kg was used to assess acute toxicity in vivo for 14 days, and repeated doses of 50, 100, and 200 mg/kg were applied for 28 days to examine sub-acute toxicity. According to the results of an acute toxicity investigation, rats given up to 2000 mg/kg did not exhibit any toxic symptoms, behavioral abnormalities, or death. Consequently, the oral hazardous dose's LD50 needs to be more than 2000 mg/mL. The safety of PHF was further validated by sub-acute toxicity experiments, which revealed no biochemical, haematological, or histological differences between rats administered with 50, 100, or 200 mg/kg and the control group (