Evidence map›Paper›PMID 40159189›Full record

ArticleThe Journal of veterinary medical science2025

Performance evaluation of an improved RAISING method for clonality analysis of bovine leukemia virus-infected cells: a collaborative study in Japan.

Tomohiro Okagawa, Naomi Nojiri, Hazuka Yoshida-Furihata, Naganori Nao, Misono Tominaga, Junko Kohara, Satoshi Gondaira, Hidetoshi Higuchi, Yohei Takeda, Haruko Ogawa and 20 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of veterinary medical science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

30 authors.

Tomohiro OkagawaDepartment of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Naomi NojiriCenter for Emergency Preparedness and Response, National Institute of Infectious Diseases, Tokyo, Japan.
Hazuka Yoshida-FurihataCenter for Emergency Preparedness and Response, National Institute of Infectious Diseases, Tokyo, Japan.
Naganori NaoDivision of International Research Promotion, International Institute for Zoonosis Control, Hokkaido University, Hokkaido, Japan.
Misono TominagaDepartment of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Junko KoharaAnimal Research Center, Agriculture Research Department, Hokkaido Research Organization, Hokkaido, Japan.
Satoshi GondairaLaboratory of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
Hidetoshi HiguchiLaboratory of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
Yohei TakedaDepartment of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Haruko OgawaDepartment of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Shinji YamadaGraduate School of Veterinary Sciences, Iwate University, Iwate, Japan.
Kenji MurakamiGraduate School of Veterinary Sciences, Iwate University, Iwate, Japan.
Yasunori SuzukiLaboratory of Animal Hygiene, Kitasato University School of Veterinary Medicine, Aomori, Japan.
Shinji TakaiLaboratory of Animal Hygiene, Kitasato University School of Veterinary Medicine, Aomori, Japan.
Masaki MaezawaLaboratory of OSG Veterinary Science for Global Disease Management, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Hisashi InokumaLaboratory of OSG Veterinary Science for Global Disease Management, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Kaori ShimizuLaboratory of Food and Environmental Hygiene, Joint Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
Yasuo InoshimaLaboratory of Food and Environmental Hygiene, Joint Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
Tatsufumi UsuiAvian Zoonosis Research Center, Faculty of Agriculture, Tottori University, Tottori, Japan.
Michihito TagawaDepartment of Veterinary Science, Okayama University of Science, Ehime, Japan.
Mari YamamotoCenter for Animal Disease Control, University of Miyazaki, Miyazaki, Japan.
Hirohisa MekataCenter for Animal Disease Control, University of Miyazaki, Miyazaki, Japan.
Mana EsakiJoint Graduate School of Veterinary Medicine, Kagoshima University, Kagoshima, Japan.
Makoto OzawaJoint Graduate School of Veterinary Medicine, Kagoshima University, Kagoshima, Japan.
Takahiro MatsudairaBiotechnological Research Support Division, FASMAC Co., Ltd., Kanagawa, Japan.
Naoya MaekawaDepartment of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Shiro MurataDepartment of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Kazuhiko OhashiDepartment of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Masumichi SaitoCenter for Emergency Preparedness and Response, National Institute of Infectious Diseases, Tokyo, Japan.
Satoru KonnaiDepartment of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bovine leukemia virus (BLV), a retrovirus that is widespread worldwide, causes enzootic bovine leukosis (EBL), a B-cell leukemia/lymphoma with a poor prognosis that ultimately results in death. In Japan, the number of cattle infected with this virus is increasing, and it is estimated more than 35% of cattle are currently infected. Since no vaccines or treatments against BLV infection are currently available, it is important to establish a method of early diagnosis for EBL to reduce economic losses caused by the disposal of EBL cattle in Japan, where a large number of expensive beef cattle are raised. We previously developed Rapid Amplification of the Integration Site without Interference by Genomic DNA Contamination (RAISING), a cost-effective, rapid, and sensitive method for the clonality analysis of BLV-infected cells. Despite its usefulness for the early diagnosis of EBL, RAISING had drawbacks preventing its practical application. Here, we report the development of an improved method, RAISING ver.2, and its performance. Compared to BLV clonality analysis using the previous method, RAISING ver.2 was found to maintain high accuracy and reproducibility despite its simplification. Moreover, its performance was also validated in a multicenter validation study. Taken together, our results strongly suggest that RAISING ver.2 can be fully utilized in clinical practice. Successful commercialization of a RAISING test kit could overcome the concerns of livestock farmers suffering from EBL, thereby promoting a stable supply of Japanese beef, both domestically and internationally.

Indexed as

Enzootic Bovine LeukosisLeukemia Virus, BovineAnimalsCattleJapanPolymerase Chain ReactionReproducibility of Resultsbovine leukemia virusclonality analysisenzootic bovine leukosismulticenter interlaboratory validationRAISING

Identifiers

PMID40159189
PMCPMC12150872

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.