Evidence map›Paper›PMID 40159929›Full record

ArticleJournal of applied toxicology : JAT2025

Safety Evaluation of Serendipity Berry Sweet Protein From Komagataella phaffii.

Yael Lifshitz, Shira Paz, Rotem Saban, Inbar Zuker, Hagay Shmuely, Katy Gorshkov, Jwar Meetro, Shahrzad Tafazoli, Trung Vo, Gabriela Amiram and 3 more

Abstract read
In one paragraph

Article in Journal of applied toxicology : JAT, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. [Robotic gastrectomy: Research progress and practical challenges].Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2026
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yael LifshitzAmai Proteins Ltd., Rehovot, Israel.
Shira PazAmai Proteins Ltd., Rehovot, Israel.
Rotem SabanAmai Proteins Ltd., Rehovot, Israel.
Inbar ZukerAmai Proteins Ltd., Rehovot, Israel.
Hagay ShmuelyAmai Proteins Ltd., Rehovot, Israel.
Katy GorshkovAmai Proteins Ltd., Rehovot, Israel.
Jwar MeetroIntertek Health Sciences Inc., Mississauga, Ontario, Canada.ORCID 0009-0003-6003-0455
Shahrzad TafazoliIntertek Health Sciences Inc., Mississauga, Ontario, Canada.
Trung VoIntertek Health Sciences Inc., Mississauga, Ontario, Canada.
Gabriela AmiramLaboratory of Chemistry of Foods and Bioactives, Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, Israel.
Carmit Shani LeviLaboratory of Chemistry of Foods and Bioactives, Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, Israel.
Uri LesmesLaboratory of Chemistry of Foods and Bioactives, Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, Israel.
Ilan SamishAmai Proteins Ltd., Rehovot, Israel.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Serendipity Berry Sweet Protein (sweelin) is a novel hyper-sweet thermophilic protein designed using Artificial Intelligence Computational Protein Design (AI-CPD) to improve the stability and sensory profile of the protein found in serendipity berry (Dioscoreophyllum cumminsii). sweelin is produced through precision fermentation by expression in Komagataella phaffii. The safety of sweelin was investigated through an evaluation of its genotoxicity, mutagenicity, systemic toxicity and digestibility potential in in vitro and in vivo models. sweelin was not genotoxic in in vitro reverse mutation and mammalian micronucleus assays and was not associated with systemic toxicity in a 90-day dietary toxicity study in rats. The no-observed-adverse-effect level for sweelin in Sprague Dawley rats was established as 14,300 ppm, the highest dose tested. This dose level corresponds to dietary intakes of 838.3 and 946.0 mg/kg body weight/day in male and female rats, respectively. sweelin was demonstrated to be readily digestible in an in vitro semi-dynamic model of the gastrointestinal tract. The results support the safety of sweelin as a food ingredient for sweetening purposes.

Indexed as

FruitAnimalsFemaleMaleMutagenicity TestsNo-Observed-Adverse-Effect LevelRatsRats, Sprague-Dawleydigestibilitygenotoxicitymonellinproteinsafety assessmentsweetenertoxicology

Identifiers

PMID40159929
PMCPMC12209738

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.