Evidence map›Paper›PMID 40161805›Full record

ArticlebioRxiv : the preprint server for biology2025

The sialome of the retina, alteration in age-related macular degeneration (AMD) pathology and potential impacts on Complement Factor H.

Jaclyn Swan, Christopher B Toomey, Max Bergstrand, Hector A Cuello, Jesse Robie, Hai Yu, Yue Yuan, Anoopjit Singh Kooner, Xi Chen, Jutamas Shaughnessy and 3 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Jaclyn SwanDepartment of Pathology, University of California San Diego, La Jolla, California, USA.ORCID 0000-0003-1633-9239
Christopher B ToomeyShiley Eye Institute, Viterbi Family Department of Ophthalmology University of California San Diego, La Jolla, California, USA.ORCID 0000-0003-0341-7787
Max BergstrandDepartment of Pathology, University of California San Diego, La Jolla, California, USA.
Hector A CuelloGlycobiology Research and Training Center, University of California San Diego, La Jolla, California, USA.ORCID 0000-0002-5304-7545
Jesse RobieDepartment of Pathology, University of California San Diego, La Jolla, California, USA.
Hai YuDepartment of Chemistry, University of California Davis, Davis, California, USA.ORCID 0000-0002-4378-0532
Yue YuanDepartment of Chemistry, University of California Davis, Davis, California, USA.ORCID 0000-0003-3524-0576
Anoopjit Singh KoonerDepartment of Chemistry, University of California Davis, Davis, California, USA.ORCID 0000-0003-1747-5689
Xi ChenDepartment of Chemistry, University of California Davis, Davis, California, USA.ORCID 0000-0002-3160-614X
Jutamas ShaughnessyDivision of Infectious Diseases and Immunology, University of Massachusetts Chan Medical School, Worcester, Massachusetts, USA.ORCID 0000-0003-0832-9954
Sanjay RamDivision of Infectious Diseases and Immunology, University of Massachusetts Chan Medical School, Worcester, Massachusetts, USA.ORCID 0000-0003-0058-9532
Ajit VarkiGlycobiology Research and Training Center, University of California San Diego, La Jolla, California, USA.ORCID 0000-0002-2206-975X
Pascal GagneuxDepartment of Pathology, University of California San Diego, La Jolla, California, USA.ORCID 0000-0001-9599-9838

Funding

Supplement: Exploring the biology of O-acetyl sialic acids using stable synthetic mimicsR01AI130684 · NIAID · UNIVERSITY OF CALIFORNIA AT DAVIS · PI CHEN, XI, VARKI, AJIT P · 2017 to 2020
$2.3M
Role of Bruch Membrane Heparan Sulfate in Drusenogenesis in Age-Related Macular DegenerationK08EY035322 · NEI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Christopher B Toomey · 2024 to 2026
$764k
NEI NIH HHS K08 EY035322NIAID NIH HHS R01 AI130684
6 · The paper itself

Abstract

Purpose: Little is known about sialic acids of the human retina, despite their integral role in self/non-self-discrimination by complement factor H (CFH), the alternative complement pathway inhibitor. Methods: A custom sialoglycan microarray was used to characterize the sialic acid-binding specificity of native CFH or recombinant molecules where IgG Fc was fused to CFH domains 16-20 (contains a sialic acid-binding site), domains 6-7 (contains a glycosaminoglycan-binding site) or the CFH-related proteins (CFHRs) 1 and 3. We analyzed macular and peripheral retinal tissue from post-mortem ocular globes for amount, type, and presentation (glycosidic linkage type) of sialic acid in individuals with age-related macular degeneration (AMD) and age-matched controls using fluorescent lectins and antibodies to detect sialic acid and endogenous CFH. Released sialic acids from neural retina, retinal pigmented epithelium (RPE) cells and the Bruch's membrane (BrM) were labelled with 1,2-diamino-4,5-methylenedioxybenzene-2HCl (DMB), separated and quantified by high-performance liquid chromatography (DMB-HPLC). Results: Both native CFH and the recombinant CFH domains 16-20 recognized Neu5Ac and Neu5Gc that is α2-3-linked to the underlying galactose. 4- Conclusions: The sialome of the human retina is altered in AMD. This can affect CFH binding and consequently, alternative complement pathway regulation.

Identifiers

PMID40161805
PMCPMC11952305

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.