ArticleNature neuroscience2025
EPSILON: a method for pulse-chase labeling to probe synaptic AMPAR exocytosis during memory formation.
Article in Nature neuroscience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- CLOK: a chemigenetic multicolor labeling system to visualize neuronal birthdate and circuit integration.Nature neuroscience · 2026Article
- Discerning protein pools by selective staining with self-labeling tags.FEBS letters · 2026Review
- Article
- Engram Synapses and Synapse Dynamics in Memory Processing.Journal of neurochemistry · 2026Review
- Activity drives local CaMKII synthesis and subcellular localization via autophosphorylation-dependent pathways.bioRxiv : the preprint server for biology · 2025Article
- Article
- Distinct spatial distribution of potentiated dendritic spines in encoding- and recall-activated hippocampal neurons.Frontiers in molecular neuroscience · 2025Article
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Authors and funding
9 authors.
Funding
Abstract
A tool to map changes in synaptic strength during a defined time window could provide powerful insights into the mechanisms of learning and memory. Here we developed a technique, Extracellular Protein Surface Labeling in Neurons (EPSILON), to map α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) exocytosis in vivo by sequential pulse-chase labeling of surface AMPARs with membrane-impermeable dyes. This approach yields synaptic-resolution maps of AMPAR exocytosis, a proxy for synaptic potentiation, in genetically targeted neurons during memory formation. In mice undergoing contextual fear conditioning, we investigated the relationship between synapse-level AMPAR exocytosis in CA1 pyramidal neurons and cell-level expression of the immediate early gene product cFos, a frequently used marker of engram neurons. We observed a strong correlation between AMPAR exocytosis and cFos expression, suggesting a synaptic mechanism for the association of cFos expression with memory engrams. The EPSILON technique is a useful tool for mapping synaptic plasticity and may be extended to investigate trafficking of other transmembrane proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.