Evidence map›Paper›PMID 40166232›Full record

ArticlebioRxiv : the preprint server for biology2025

Spatial Transcriptomics Identifies Immune-Stromal Niches Associated with Cancer in Adult Dermatomyositis.

Ksenia S Anufrieva, Neda Shahriari, Ce Gao, Rochelle L Castillo, Jessica Liu, Sean Prell, Shideh Kazerounian, Khashayar Afshari, Anastasia N Kazakova, Erin Theisen and 7 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

17 authors.

Ksenia S AnufrievaDivision of Rheumatology, Inflammation, and Immunity, Brigham and Women's Hospital at Harvard Medical School, Boston, MA, USA.
Neda ShahriariDepartment of Dermatology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Ce GaoDivision of Rheumatology, Inflammation, and Immunity, Brigham and Women's Hospital at Harvard Medical School, Boston, MA, USA.
Rochelle L CastilloDepartment of Dermatology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Jessica LiuDivision of Rheumatology, Inflammation, and Immunity, Brigham and Women's Hospital at Harvard Medical School, Boston, MA, USA.
Sean PrellDivision of Rheumatology, Inflammation, and Immunity, Brigham and Women's Hospital at Harvard Medical School, Boston, MA, USA.
Shideh KazerounianDivision of Rheumatology, Inflammation, and Immunity, Brigham and Women's Hospital at Harvard Medical School, Boston, MA, USA.
Khashayar AfshariDepartment of Dermatology, UMass Chan Medical School, Worcester, MA, USA.
Anastasia N KazakovaLopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.
Erin TheisenDivision of Rheumatology, Inflammation, and Immunity, Brigham and Women's Hospital at Harvard Medical School, Boston, MA, USA.
Teresa BowmanDepartment of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Avery LaChanceDepartment of Dermatology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Kimberly HashemiDepartment of Dermatology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Ilya KorsunskyDivision of Genetics, Brigham and Women's Hospital at Harvard Medical School, Boston, MA, USA.
Mehdi RashighiDepartment of Dermatology, UMass Chan Medical School, Worcester, MA, USA.
Ruth Ann VleugelsDepartment of Dermatology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Kevin WeiDivision of Rheumatology, Inflammation, and Immunity, Brigham and Women's Hospital at Harvard Medical School, Boston, MA, USA.

Funding

Pathogenic fibroblast differentiation in rheumatoid arthritisK08AR077037 · NIAMS · BRIGHAM AND WOMEN'S HOSPITAL · PI WEI, KEVIN S · 2020 to 2024
$799k
Using high dimensional molecular data to decipher gene dynamics underlying pathogenic synovial fibroblastsK01AR078355 · NIAMS · BRIGHAM AND WOMEN'S HOSPITAL · PI KORSUNSKY, ILYA · 2021 to 2025
$442k
NIAMS NIH HHS K01 AR078355NIAMS NIH HHS K08 AR077037
6 · The paper itself

Abstract

Adult-onset dermatomyositis (DM) is an autoimmune inflammatory myopathy with distinct cutaneous manifestations and a strong malignancy association. Through comparative analysis with cutaneous lupus erythematosus (CLE), our integrated spatial and single-cell transcriptomics analysis revealed unique immune and stromal niches associated with DM subtypes. Unexpectedly, we found an association between cancer-associated DM skin lesions and the presence of dispersed immune infiltrates enriched with macrophages, CD8+ T cells, plasma cells, and B cells with preserved vascular architecture. In contrast, non-cancer associated DM skin exhibited dense myeloid cell infiltrates, including neutrophils, monocytes, and macrophages, with elevated expression of IL1B and CXCL10 localized near injured vascular endothelia. Cytokines produced by these myeloid infiltrates together with local tissue hypoxia triggered dramatic stromal remodeling, leading to loss of vascular-associated fibroblasts. In addition to the CXCL10+ myeloid signature, non-cancer-associated DM skin with pDC presence showed the emergence of specific cellular pairs: PD-L1-expressing mregDCs and activated Tregs expressing NFKB2 and TNF receptors. While both DM and CLE showed strong interferon signatures, DM uniquely displayed IFN-β expression. Together, our study provides the first comprehensive spatial mapping of immune and stromal cells in adult-onset DM.

Indexed as

Cancer-Associated and Non-Cancer-Associated DermatomyositisCutaneous Lupus ErythematosusHypoxiaIL1B ResponseImmune AggregatesInterferon ResponseNeutrophilsSingle CellSpatial Single CellStromal Remodeling

Identifiers

PMID40166232
PMCPMC11957040

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.