Evidence map›Paper›PMID 40167911›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Targeted Absolute Quantification of Protein Biomarkers in Cerebrospinal Fluid by FastCAT.

Ignacy Rzagalinski, Viditha Rao, Aliona Bogdanova, Lena Hersemann, Andrej Shevchenko

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ignacy RzagalinskiMax Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Viditha RaoMax Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Aliona BogdanovaMax Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Lena HersemannMax Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Andrej ShevchenkoMax Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany. shevchenko@mpi-cbg.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Absolute (molar) quantification of proteins in cerebrospinal fluid (CSF) provides reference values and ranges of their physiological variation for perspective biomarkers across a broad spectrum of neurological disorders. FastCAT (for Fast-track QconCAT) method employs targeted mass spectrometry and multiple recombinant stable-isotope labeled chimeric proteins (CPs) as internal calibrants for the accurate and consistent determination of molar concentrations of dozens of CSF proteins. No purification or external quantification of CPs is required. We demonstrate that FastCAT, powered by parallel reaction monitoring (PRM) detection of multiple quantotypic peptides per target proteins, can quantify the molar concentration of neurological biomarkers from neat CSF over a dynamic range of more than 1000-fold, with high precision (coefficient of variation <20%) and low detection limits (<1 nmol/L) in a single LC-MS/MS analysis.

Indexed as

BiomarkersCerebrospinal Fluid ProteinsProteomicsChromatography, LiquidHumansIsotope LabelingTandem Mass SpectrometryBiomarkersCerebrospinal Fluid ProteinsAbsolute quantificationCerebrospinal fluidFastCATProtein biomarkersTargeted proteomics

Identifiers

PMID40167911

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.