Evidence map›Paper›PMID 40182494›Full record

ArticleRSC advances2025

Identification of acrylamide-based covalent inhibitors of SARS-CoV-2 (SCoV-2) Nsp15 using high-throughput screening and machine learning.

Teena Bajaj, Babak Mosavati, Lydia H Zhang, Mohammad S Parsa, Huanchen Wang, Evan M Kerek, Xueying Liang, Seyed Amir Tabatabaei Dakhili, Eddie Wehri, Silin Guo and 10 more

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RSC advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

20 authors.

Teena BajajGraduate Program of Comparative Biochemistry, University of California, Berkeley Berkeley CA USA bajajtiya@berkeley.edu.
Babak MosavatiInnovative Genomics Institute, University of California, Berkeley Berkeley CA USA bmosavati@berkeley.edu.
Lydia H ZhangGraduate Program of Molecular Toxicology, University of California, Berkeley Berkeley CA USA.
Mohammad S ParsaDepartment of Applied Science and Technology, University of California, Berkeley CA USA.ORCID https://orcid.org/0000-0002-3416-739X
Huanchen WangSignal Transduction Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services North Carolina USA.
Evan M KerekDepartment of Pharmacology, Li Ka Shing Institute of Virology, University of Alberta Edmonton Alberta Canada.
Xueying LiangDepartment of Physiological Sciences, College of Veterinary Medicine, Oklahoma Center for Respiratory and Infectious Disease, Oklahoma State University Oklahoma USA nmurthy@berkeley.edu.
Seyed Amir Tabatabaei DakhiliFaculty of Pharmacy and Pharmaceutical Sciences, University of Alberta Edmonton Alberta Canada.
Eddie WehriThe Henry Wheeler Center for Emerging and Neglected Diseases, University of California, Berkeley Berkeley CA USA.
Silin GuoDepartment of Chemistry, University of California, Berkeley Berkeley CA USA.
Rushil N DesaiDepartment of Bioengineering, University of California, Berkeley Berkeley CA USA.
Lauren M OrrDepartment of Chemistry, University of California, Berkeley Berkeley CA USA.ORCID https://orcid.org/0009-0003-4326-5789
Mohammad R K MofradDepartment of Mechanical Engineering, University of California Berkeley CA USA.ORCID https://orcid.org/0000-0001-7004-4859
Julia SchaletzkyThe Henry Wheeler Center for Emerging and Neglected Diseases, University of California, Berkeley Berkeley CA USA.
John R UssherFaculty of Pharmacy and Pharmaceutical Sciences, University of Alberta Edmonton Alberta Canada.
Xufang DengDepartment of Physiological Sciences, College of Veterinary Medicine, Oklahoma Center for Respiratory and Infectious Disease, Oklahoma State University Oklahoma USA nmurthy@berkeley.edu.
Robin StanleySignal Transduction Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services North Carolina USA.
Basil P HubbardDepartment of Pharmacology, Li Ka Shing Institute of Virology, University of Alberta Edmonton Alberta Canada.ORCID https://orcid.org/0000-0003-4353-9674
Daniel K NomuraDepartment of Chemistry, University of California, Berkeley Berkeley CA USA.ORCID https://orcid.org/0000-0003-1614-8360
Niren MurthyDepartment of Physiological Sciences, College of Veterinary Medicine, Oklahoma Center for Respiratory and Infectious Disease, Oklahoma State University Oklahoma USA nmurthy@berkeley.edu.ORCID https://orcid.org/0000-0002-7815-7337

Funding

Structural and Functional Characterization of the SARS-CoV-2 Endoribonuclease Nsp15ZIAES103340 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI STANLEY, ROBIN · 2020 to 2025
$3.6M
Gene editing in the brain with CRISPR-PEGR01MH125979 · NIMH · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI LEE, HYE YOUNG, MURTHY, NIREN · 2021 to 2025
$3.3M
Imaging bacterial infections with 2nd generation maltodextrinsR01EB029320 · NIBIB · EMORY UNIVERSITY · PI GOODMAN, MARK MYRON, MURTHY, NIREN · 2020 to 2023
$2.7M
Development of a Nanoparticle-Based Gene Editing Technology for Neurological ApplicationsUG3NS115599 · NINDS · OHIO STATE UNIVERSITY · PI BANKIEWICZ, KRYSTOF S, MURTHY, NIREN · 2019 to 2021
$2.3M
Single-Cell Transcriptomics of Non-Activated Latently Infected T cells Isolated from HIV+ Drug UsersR61DA048444 · NIDA · J. DAVID GLADSTONE INSTITUTES · PI OTT, MELANIE MARIA · 2019 to 2021
$2.0M
Intramural NIH HHS ZIA ES103340NIBIB NIH HHS R01 EB029320NIDA NIH HHS R61 DA048444NIMH NIH HHS R01 MH125979NINDS NIH HHS UG3 NS115599
6 · The paper itself

Abstract

Non-structural protein 15 (Nsp15) is a SARS-CoV-2 (SCoV-2) endoribonuclease and is a promising target for drug development because of its essential role in evading the host immune system. However, developing inhibitors against Nsp15 has been challenging due to its structural complexity and large RNA binding surface. In this report, we screened a 2640 acrylamide-based compound library against Nsp15 and identified 10 fragments that reacted with cysteine residues on Nsp15 and inhibited its endoribonuclease activity with IC

Identifiers

PMID40182494
PMCPMC11966100

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.