Evidence map›Paper›PMID 40192089›Full record

ArticleInternational wound journal2025

Effectiveness of quality and quantity mononuclear cells for enhancing wound healing in diabetic ischemic limb animal model.

Pongpol Sriphan, Nuttapol Chruewkamlow, Chutipon Sathan-Ard, Phakawan Phutthakunphithak, Somponnat Sampattavanich, Tauangtham Anekpuritanang, Romgase Sakamula, Thanaphon Likhityungyuen, Chumpol Wongwanit, Chanean Ruangsetakit and 1 more

Abstract read
In one paragraph

Article in International wound journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. A Marine-Derived Steroid fromMarine drugs · 2025
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Pongpol SriphanDivision of Vascular Surgery, Department of Surgery, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Nuttapol ChruewkamlowResearch Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Chutipon Sathan-ArdResearch Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Phakawan PhutthakunphithakResearch Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Somponnat SampattavanichDepartment of Pharmacology, Faculty of Medicine Siriraj Hospital, Siriraj Center of Research Excellence for Systems Pharmacology, Mahidol University, Bangkok, Thailand.
Tauangtham AnekpuritanangDepartment of Pharmacology, Faculty of Medicine Siriraj Hospital, Siriraj Center of Research Excellence for Systems Pharmacology, Mahidol University, Bangkok, Thailand.
Romgase SakamulaDepartment of Pharmacology, Faculty of Medicine Siriraj Hospital, Siriraj Center of Research Excellence for Systems Pharmacology, Mahidol University, Bangkok, Thailand.
Thanaphon LikhityungyuenDepartment of Pharmacology, Faculty of Medicine Siriraj Hospital, Siriraj Center of Research Excellence for Systems Pharmacology, Mahidol University, Bangkok, Thailand.
Chumpol WongwanitDivision of Vascular Surgery, Department of Surgery, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Chanean RuangsetakitDivision of Vascular Surgery, Department of Surgery, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Nuttawut SermsathanasawadiDivision of Vascular Surgery, Department of Surgery, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.

Funding

Mahidol University FF-035/2566
6 · The paper itself

Abstract

This study set forth to investigate the efficacy of Quality and Quantity mononuclear cells (QQMNCs) for promoting wound healing and limb salvage in a severe ischemic wound model using diabetic mice. Female BALB/c nude mice induced with diabetes were used to create ischemic limb models in a controlled experimental design. Intramuscular injections of human QQMNCs were compared to phosphate-buffered saline (PBS) and peripheral blood mononuclear cells (PBMNCs) relative to their effects on wound healing and limb salvage. In vitro analysis demonstrated that the QQMNC group had significantly higher median percentages of CD34+ cells, CD34+CD133+ cells, CD206+ cells, and FOXP3+ cells compared to the PBMNC group (all p < 0.05), which suggests an enhanced regenerative and immunomodulatory profile. Kaplan-Meier survival analysis showed a significantly higher number of completely healed wounds in the QQMNC group than in the PBMNC group (p = 0.044). The histological evaluation showed that the QQMNC group had a significantly thinner epithelial thickness than the PBMNC (p = 0.032) and PBS groups (p = 0.002), and a significantly greater T cell density than the PBS group (p = 0.033), which suggests more efficient tissue repair. Moreover, the QQMNC group exhibited the highest percentage of minor tissue loss (57% for forefoot and toe gangrene), and the lowest incidence of severe limb loss (0% for lower leg gangrene). The findings of this study highlight the effectiveness of QQMNCs for promoting wound healing and limb salvage in diabetic ischemic animal model; however, clinical trials are needed to further assess their efficacy in this clinical context.

Indexed as

Diabetes Mellitus, ExperimentalIschemiaLeukocytes, MononuclearWound HealingAnimalsDisease Models, AnimalFemaleHumansLimb SalvageMiceMice, Inbred BALB CMice, Nudediabetic ischemic limb animal modeleffectivenessquality and quantity mononuclear cellswound healing

Identifiers

PMID40192089
PMCPMC11973723

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.