Evidence map›Paper›PMID 40195317›Full record

ArticleNature communications2025

dFLASH; dual FLuorescent transcription factor activity sensor for histone integrated live-cell reporting and high-content screening.

Timothy P Allen, Alison E Roennfeldt, Moganalaxmi Reckdharajkumar, Adrienne E Sullivan, Miaomiao Liu, Ronald J Quinn, Darryl L Russell, Daniel J Peet, Murray L Whitelaw, David C Bersten

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In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Timothy P AllenSchool of Biological Sciences, University of Adelaide, Adelaide, SA, Australia.ORCID http://orcid.org/0000-0002-8190-2334
Alison E RoennfeldtSchool of Biological Sciences, University of Adelaide, Adelaide, SA, Australia.
Moganalaxmi ReckdharajkumarSchool of Biological Sciences, University of Adelaide, Adelaide, SA, Australia.ORCID http://orcid.org/0000-0002-9136-2810
Adrienne E SullivanAdelaide Centre for Epigenetics, School of Biomedicine, Faculty of Health and Medical Sciences, The University of Adelaide, Adelaide, SA, Australia.ORCID http://orcid.org/0000-0003-3967-892X
Miaomiao LiuGriffith Institute for Drug Discovery, Griffith University, Brisbane, QLD, Australia.ORCID http://orcid.org/0000-0003-0930-3617
Ronald J Quinn *Griffith Institute for Drug Discovery, Griffith University, Brisbane, QLD, Australia.ORCID http://orcid.org/0000-0002-4022-2623
Darryl L Russell *Robinson Research Institute, University of Adelaide, Adelaide, SA, Australia.
Daniel J Peet *School of Biological Sciences, University of Adelaide, Adelaide, SA, Australia.ORCID http://orcid.org/0000-0002-6085-8936
Murray L Whitelaw *School of Biological Sciences, University of Adelaide, Adelaide, SA, Australia.
David C BerstenSchool of Biological Sciences, University of Adelaide, Adelaide, SA, Australia. david.bersten@adelaide.edu.au.ORCID http://orcid.org/0000-0003-4952-959X

Funding

Bill and Melinda Gates Foundation (Bill & Melinda Gates Foundation) OPP1171844, INV-001616Gates Foundation INV-001616
6 · The paper itself

Abstract

Live-cell transcription factor (TF) activity reporting is crucial for synthetic biology, drug discovery and functional genomics. Here we present dFLASH (dual FLuorescent transcription factor Activity Sensor for Histone-integrated live-cell reporting), a modular, genome-integrated TF sensor. dFLASH homogeneously and specifically detects endogenous Hypoxia Inducible Factor (HIF) and Progesterone Receptor (PGR) activities, as well as coactivator recruitment to synthetic TFs. The dFLASH system produces dual-color nuclear fluorescence, enabling normalized, dynamic, live-cell TF activity sensing with strong signal-to-noise ratios and robust screening performance (Z' = 0.61-0.74). We validate dFLASH for functional genomics and drug screening, demonstrating HIF regulation via CRISPRoff and application to whole-genome CRISPR KO screening. Additionally, we apply dFLASH for drug discovery, identifying HIF pathway modulators from a 1600-compound natural product library using high-content imaging. Together, this versatile platform provides a powerful tool for studying TF activity across diverse applications.

Indexed as

Biosensing TechniquesHistonesTranscription FactorsCRISPR-Cas SystemsDrug DiscoveryHEK293 CellsHigh-Throughput Screening AssaysHumansReceptors, ProgesteroneHistonesReceptors, ProgesteroneTranscription Factors

Identifiers

PMID40195317
PMCPMC11977238

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.