Evidence map›Paper›PMID 40196590›Full record

ArticlebioRxiv : the preprint server for biology2025

Glutamate indicators with increased sensitivity and tailored deactivation rates.

Abhi Aggarwal, Adrian Negrean, Yang Chen, Rishyashring Iyer, Daniel Reep, Anyi Liu, Anirudh Palutla, Michael E Xie, Bryan J MacLennan, Kenta M Hagihara and 24 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

34 authors.

Abhi AggarwalAllen Institute for Neural Dynamics, Seattle, Washington, USA.ORCID 0000-0002-6336-566X
Adrian NegreanAllen Institute for Neural Dynamics, Seattle, Washington, USA.
Yang ChenInstitute of Neuroscience and Munich Cluster for Systems Neurology, Technical University of Munich, Munich, Germany.
Rishyashring IyerDepartment of Physics, University of California, San Diego, La Jolla, California, USA.
Daniel ReepJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.ORCID 0009-0009-7638-9538
Anyi LiuUniversity College London, Gower St, London, United Kingdom.
Anirudh PalutlaJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
Michael E XieJohns Hopkins University, Baltimore, MD, USA.ORCID 0000-0002-4026-9181
Bryan J MacLennanAllen Institute for Neural Dynamics, Seattle, Washington, USA.ORCID 0009-0000-2619-1608
Kenta M HagiharaAllen Institute for Neural Dynamics, Seattle, Washington, USA.ORCID 0000-0003-0064-0852
Lucas W KinseyAllen Institute for Neural Dynamics, Seattle, Washington, USA.
Julianna L SunNeuronal Cell Biology Division, Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, USA.ORCID 0000-0002-5172-4709
Pantong YaoDepartment of Neurosciences, University of California, San Diego, La Jolla, California, USA.
Jihong ZhengJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
Arthur TsangJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
Getahun TsegayeJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
Yonghai ZhangInstitute of Neuroscience and Munich Cluster for Systems Neurology, Technical University of Munich, Munich, Germany.
Ronak H PatelJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
Benjamin J ArthurJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
Julien HiblotMax Planck Institute for Medical Research, Heidelberg, Germany.ORCID 0000-0002-7883-8079
Philipp LeippeCeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Wien, Austria.
Miroslaw TarnawskiMax Planck Institute for Medical Research, Heidelberg, Germany.ORCID 0000-0001-5297-5668
Jonathan S MarvinJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
Jason D VeveaNeuronal Cell Biology Division, Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, USA.ORCID 0000-0002-3068-973X
Srinivas C TuragaJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.ORCID 0000-0003-3247-6487
Alison G TeboJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.ORCID 0000-0003-0788-5617
Matteo CarandiniUniversity College London, Gower St, London, United Kingdom.
L Federico RossiCenter for Neuroscience and Cognitive Systems, Italian Institute of Technology, Rovereto, Italy.ORCID 0000-0001-5831-4860
David KleinfeldDepartment of Neurobiology, University of California, San Diego, La Jolla, California, USA.
Arthur KonnerthInstitute of Neuroscience and Munich Cluster for Systems Neurology, Technical University of Munich, Munich, Germany.
Karel SvobodaAllen Institute for Neural Dynamics, Seattle, Washington, USA.
Glenn C TurnerJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.ORCID 0000-0002-5341-2784
Jeremy HassemanJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.ORCID 0000-0002-0096-7321
Kaspar PodgorskiAllen Institute for Neural Dynamics, Seattle, Washington, USA.ORCID 0000-0002-0374-2005

Funding

Top-down and bottom-up signals for flexible orofacial behaviorsU19NS137920 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Beth Friedman · 2024 to 2026
$15.9M
Optimization of genetically encoded voltage and neurotransmitter indicators for multiwavelength in vivo analysis of brain circuitsUM1MH136462 · NIMH · MAX PLANCK FLORIDA CORPORATION · PI Michael Z. Lin, Kaspar Podgorski · 2024 to 2026
$6.3M
Optimization of Calcium and RNA multiplexed activity imaging for highly parallelized evaluation of cell type functions in deep-brain structuresU01NS126054 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CUI, MENG, STERNSON, SCOTT M · 2022 to 2025
$3.0M
Measuring input-output operations of cortical neurons with large-scale neurotransmitter imagingDP2NS136990 · NINDS · ALLEN INSTITUTE · PI Kaspar Podgorski · 2023 to 2026
$2.5M
Imaging the molecular constituents of the brain vasculature and lymphatic connectomeU24EB028942 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI KLEINFELD, DAVID · 2019 to 2024
$1.8M
CRCNS : Thalamocortical vs. recurrent connectivity in active sensation of vibrissa touchR01NS143141 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI David Kleinfeld · 2025 to 2026
$551k
STUDYING SINGLE NEURON COMPUTATIONS WITHIN BRAIN-WIDE CIRCUITSF30MH138009 · NIMH · JOHNS HOPKINS UNIVERSITY · PI Michael Everest Xie · 2024 to 2026
$164k
NIBIB NIH HHS U24 EB028942NIMH NIH HHS F30 MH138009NIMH NIH HHS UM1 MH136462NINDS NIH HHS DP2 NS136990NINDS NIH HHS R01 NS143141NINDS NIH HHS U01 NS126054NINDS NIH HHS U19 NS137920
6 · The paper itself

Abstract

Identifying the input-output operations of neurons requires measurements of synaptic transmission simultaneously at many of a neuron's thousands of inputs in the intact brain. To facilitate this goal, we engineered and screened 3365 variants of the fluorescent protein glutamate indicator iGluSnFR3 in neuron culture, and selected variants in the mouse visual cortex. Two variants have high sensitivity, fast activation (< 2 ms) and deactivation times tailored for recording large populations of synapses (iGluSnFR4s, 153 ms) or rapid dynamics (iGluSnFR4f, 26 ms). By imaging action-potential evoked signals on axons and visually-evoked signals on dendritic spines, we show that iGluSnFR4s/4f primarily detect local synaptic glutamate with single-vesicle sensitivity. The indicators detect a wide range of naturalistic synaptic transmission, including in the vibrissal cortex layer 4 and in hippocampal CA1 dendrites. iGluSnFR4 increases the sensitivity and scale (4s) or speed (4f) of tracking information flow in neural networks

Identifiers

PMID40196590
PMCPMC11974752

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.