Evidence map›Paper›PMID 40198566›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

A Protocol for High-efficiency Transformation and Genome Editing in Elite Wheat Cultivars.

Karminderbir Kaur, Akshaya K Biswal, Rajvinder Kaur, Mehtab- Singh, Kanwarpal S Dhugga, Jaswinder Singh

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Karminderbir KaurDepartment of Plant Science, McGill University, Ste Anne de Bellevue, QC, Canada.
Akshaya K BiswalInternational Maize and Wheat Improvement Center (CIMMYT), Ciudad dee México, Mexico.
Rajvinder KaurDepartment of Plant Science, McGill University, Ste Anne de Bellevue, QC, Canada.
Mehtab- SinghDepartment of Plant Science, McGill University, Ste Anne de Bellevue, QC, Canada.
Kanwarpal S DhuggaInternational Maize and Wheat Improvement Center (CIMMYT), Ciudad dee México, Mexico.
Jaswinder SinghDepartment of Plant Science, McGill University, Ste Anne de Bellevue, QC, Canada. jaswinder.singh@mcgill.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

CRISPR-Cas9-based editing offers a simple yet efficient method for introducing targeted mutations in the genes of interest. Low efficiency of transformation and a lack of reproducibility in editing genes in elite wheat cultivars are the bottlenecks in improving simply inherited traits. We describe a high-efficiency transformation protocol for editing elite wheat cultivars using JD633-GRF4-GIF1 CRISPR vector. This vector reduces the regeneration time of transformants to less than 90 days and enables transformation of elite cultivars.

Indexed as

CRISPR-Cas SystemsGene EditingGenome, PlantTransformation, GeneticTriticumGenetic VectorsPlants, Genetically ModifiedCRISPR-Cas9Elite cultivarsGene editingGene gunParticle bombardmentTransformationWheat gene editing

Identifiers

PMID40198566

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.