ArticleApplied and environmental microbiology2025
Single-cell pigment analysis of phototrophic and phyllosphere bacteria using simultaneous detection of Raman and autofluorescence spectra.
Article in Applied and environmental microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Microbes produce various types of pigments that are essential for their biological activities. Microbial pigments are important for humans because they are used in the food industry and medicine. The visualization and evaluation of the pigment diversity of microbial cells living in natural environments will contribute not only to the understanding of their ecophysiology but also to the screening of useful microbes. Here, we demonstrate the simultaneous, nondestructive detection of the resonance Raman and autofluorescence spectra of pigments in model purple phototrophic bacteria at the single-cell level. The single-cell Raman spectra measured using confocal laser Raman microspectroscopy with 632.8 nm excitation covered the wavenumber range of 660-3,022 cm
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