Kinetic and analytical characterization of a new tropinone oxidase enzyme and its application to the simultaneous determination of the tropane alkaloids atropine and scopolamine.

Mario Domínguez, Susana de Marcos, Javier Galbán

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Article in Analytical and bioanalytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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3 authors.

Mario DomínguezAnalytical Chemistry Department, University of Zaragoza and, Instituto de Nanociencia y Materiales de Aragón (INMA), CSIC-Universidad de Zaragoza, 50009, Zaragoza, Spain.

Susana de MarcosAnalytical Chemistry Department, University of Zaragoza and, Instituto de Nanociencia y Materiales de Aragón (INMA), CSIC-Universidad de Zaragoza, 50009, Zaragoza, Spain.

Javier GalbánAnalytical Chemistry Department, University of Zaragoza and, Instituto de Nanociencia y Materiales de Aragón (INMA), CSIC-Universidad de Zaragoza, 50009, Zaragoza, Spain. jgalban@unizar.es.

Funding

Diputación General de Aragon (Research groups funding) E-25_23RMinisterio de Ciencia y Tecnología PID2022-139235OB-I00

6 · The paper itself

Abstract

A spectrophotometric enzymatic method for the determination of atropine (Atp) and scopolamine (Scp), two tropane alkaloids (TAs), has been developed. The method is based on a previous basic hydrolysis to tropine (Trp) and scopine (Sci) respectively, and a subsequent enzymatic oxidation catalyzed by a tropinone reductase 1 (TRase) using NAD as oxidant; the absorbance of NADH (340 nm) is monitored during the reaction. First, the enzyme kinetics of both substrates are studied; it is concluded that both TAs follow a compulsory order ternary complex mechanism and the Michalis-Menten constant is calculated. Then, an enzymatic method was optimized for Atp, allowing the determination of this substrate in the range from 1.1·10

Indexed as

AtropineScopolamineTropanesKineticsLimit of DetectionAtropineScopolamineTropanesChiaColorimetric enzymatic methodMichaelis–Menten kineticsNADH

Identifiers

PMID40208267

DOI10.1007/s00216-025-05856-6

PMCPMC12103354

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