Evidence map›Paper›PMID 40208490›Full record

ArticleMolecular biology reports2025

Unraveling the crucial role of UPR pathway in rat brain development.

Yan Jiang, Ajun Wang, Nian Zou, Huimin Dai, Ran Shan, Siyu Ren, Tongcui Jiang, Nan Li

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In one paragraph

Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yan JiangAnhui Medical University, Hefei, China.
Ajun WangDepartment of Neurosurgery, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Nian ZouThe Second School of Clinical Medicine, Anhui Medical University, Hefei, China.
Huimin DaiAnhui Medical University, Hefei, China.
Ran ShanAnhui Medical University, Hefei, China.
Siyu RenAnhui Medical University, Hefei, China.
Tongcui JiangDepartment of Biochemistry & Molecular Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China. tongcuij@ahmu.edu.cn.
Nan LiDepartment of Neurosurgery, The First Affiliated Hospital of USTC, Division of Life Science and Medicine, University of Science and Technology of China, Hefei, China. nslinan@163.com.

Funding

Basic and Clinical Cooperative Research Promotion Program of Anhui Medical University 2023xkjT012Key Scientific Research Foundation of the Education Department of Province Anhui 2022AH050718
6 · The paper itself

Abstract

The development of the central nervous system (CNS) is essential for the growth of an organism. The unfolded protein response (UPR) plays an important role in the occurrence and progression of CNS. Hence, to identify the changing pattern of UPR signaling molecules and their related target molecules is extremely important. Immunohistochemical (IHC) analysis was conducted to assess the expression of NeuN, a neuronal marker, within the M1 region of the cerebral cortex of wild-type (WT) rats aged different weeks. The results showed a progressive increase in the expression of NeuN in correlation with the weekly age progression of the rats. Concurrently, in the CA3 region of the hippocampus and the S1Tr area of the cerebral cortex, the levels of UPR key molecules, GRP78 and XBP1s, exhibited an upward trend in accordance with the rats' weekly age. Western blot analysis revealed an inclination toward an increase in the expression of endoplasmic reticulum (ER) stress -associated key molecules, including GRP78, ATF6, XBP1, eIF-2α, CHOP and phosphorylated eIF-2α (p-eIF-2α). Additionally, reverse transcription quantitative polymerase chain reaction (RT-qPCR) demonstrated a positive correlation between the expression of CHOP and the weekly age of the rats. These findings demonstrate that UPR signaling pathway has a certain pattern of change in the development of rat brain, suggesting that the UPR signaling pathway may play an important role in the development of the CNS, and suggesting that an applicability of XBP1s as a therapeutic candidate for childhood autism.

Indexed as

BrainUnfolded Protein ResponseActivating Transcription Factor 6AnimalsAntigens, NuclearCerebral CortexEndoplasmic Reticulum Chaperone BiPEndoplasmic Reticulum StressHeat-Shock ProteinsHippocampusMaleNerve Tissue ProteinsNeuronsRatsSignal TransductionTranscription Factor CHOPActivating Transcription Factor 6Antigens, NuclearEndoplasmic Reticulum Chaperone BiPGRP78 protein, ratHeat-Shock ProteinsNerve Tissue ProteinsRbfox3 protein, ratTranscription Factor CHOPX-Box Binding Protein 1Xbp1 protein, ratER stressRat brain developmentUnfolded protein responseXBP1s

Identifiers

PMID40208490

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.