Evidence map›Paper›PMID 40211559›Full record

ArticleSmall methods2025

Cationic Polyelectrolyte Adsorption onto Anionic Nanoparticles Analyzed with Frequency-Domain Scanning Fluorescence Correlation Spectroscopy.

Lucas Oorlynck, Lenny Van Daele, Alina Myslovska, Sarah Goddaer, Sumit Sumit, Yera Ye Ussembayev, Katrien Remaut, Iwan Moreels, Peter Dubruel, Filip Strubbe

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Small methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Lucas OorlynckDepartment of Electronics and Information Systems, Ghent University, Tech Lane Ghent Science Park - Campus A 126, Ghent, 9052, Belgium.ORCID https://orcid.org/0000-0001-9838-3713
Lenny Van DaelePolymer Chemistry and Biomaterials Group (PBM), Centre of Macromolecular Chemistry (CMaC), Department of Organic and Macromolecular Chemistry, Ghent University, Krijgslaan 281-S4, Ghent, 9000, Belgium.ORCID https://orcid.org/0000-0002-6335-395X
Alina MyslovskaPhysics and Chemistry of Nanostructures Group (PCN), Department of Chemistry, Ghent University, Krijgslaan 281-S3, Ghent, 9000, Belgium.ORCID https://orcid.org/0000-0003-0231-2959
Sarah GoddaerLaboratory for General Biochemistry and Physical Pharmacy, Department of Pharmaceutics, Ghent University, Ottergemsesteenweg 460, Ghent, 9000, Belgium.ORCID https://orcid.org/0000-0002-5376-1554
Sumit SumitDepartment of Electronics and Information Systems, Ghent University, Tech Lane Ghent Science Park - Campus A 126, Ghent, 9052, Belgium.ORCID https://orcid.org/0000-0003-2707-0466
Yera Ye UssembayevDepartment of Electronics and Information Systems, Ghent University, Tech Lane Ghent Science Park - Campus A 126, Ghent, 9052, Belgium.ORCID https://orcid.org/0000-0002-4779-9046
Katrien RemautLaboratory for General Biochemistry and Physical Pharmacy, Department of Pharmaceutics, Ghent University, Ottergemsesteenweg 460, Ghent, 9000, Belgium.ORCID https://orcid.org/0000-0002-2244-1339
Iwan MoreelsPhysics and Chemistry of Nanostructures Group (PCN), Department of Chemistry, Ghent University, Krijgslaan 281-S3, Ghent, 9000, Belgium.ORCID https://orcid.org/0000-0003-3998-7618
Peter DubruelPolymer Chemistry and Biomaterials Group (PBM), Centre of Macromolecular Chemistry (CMaC), Department of Organic and Macromolecular Chemistry, Ghent University, Krijgslaan 281-S4, Ghent, 9000, Belgium.ORCID https://orcid.org/0000-0002-5928-7726
Filip StrubbeDepartment of Electronics and Information Systems, Ghent University, Tech Lane Ghent Science Park - Campus A 126, Ghent, 9052, Belgium.ORCID https://orcid.org/0000-0003-0078-3888

Funding

Bijzonder Onderzoeksfonds UGent BOF.BAS.2022.0023.01Fonds Wetenschappelijk Onderzoek 1SA2720NFonds Wetenschappelijk Onderzoek 1SD0721NFonds Wetenschappelijk Onderzoek G037221NFonds Wetenschappelijk Onderzoek G0H7520NFonds Wetenschappelijk Onderzoek I006920N
6 · The paper itself

Abstract

An improved small-circle scanning fluorescence correlation spectroscopy (sFCS) technique is introduced by combining acousto-optical laser scanning with fitting the autocorrelation function in the frequency domain. The technique is validated using both simulation and experimental data on various fluorescent nanoparticles, including polystyrene beads, CdSe/CdS quantum dots, and lipid nanoparticles. Then, the sFCS method is used to investigate the adsorption of in-house synthesized poly(2-guanidinoethyl methacrylate) (PGUMA) polymers on polystyrene beads as a model system for polymer-coated particles in biomedical and gene delivery applications. Using the particle diffusion and illumination beam waist values obtained from our sFCS analysis, regions of polymer concentrations are identified where polymer-particle complexes remain stable. An increase in hydrodynamic size is also observed with the molecular mass of the adsorbed polymer. Beyond quantifying polymer-particle stability and hydrodynamic size, the sFCS technique offers the advantage of not requiring a time-consuming calibration step for the measurement volume, unlike standard FCS.

Indexed as

fluorescence correlation spectroscopynanoparticle agglomerationnanoparticlespolyelectrolyte adsorptionscanning microscopy

Identifiers

PMID40211559
PMCPMC12464656

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.