Evidence map›Paper›PMID 40229382›Full record

ArticleScientific reports2025

Escherichia coli type I toxin TisB exclusively controls proton depolarization following antibiotic induced DNA damage.

Tekle Airgecho Lobie, Charlotte Solum Krog, Kirsten Skarstad, Magnar Bjørås, James Alexander Booth

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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Tekle Airgecho LobieDepartment of Microbiology, University of Oslo, and Oslo University Hospital, Rikshospitalet, Oslo, Norway.
Charlotte Solum KrogDepartment of Microbiology, University of Oslo, and Oslo University Hospital, Rikshospitalet, Oslo, Norway.
Kirsten SkarstadDepartment of Microbiology, University of Oslo, and Oslo University Hospital, Rikshospitalet, Oslo, Norway.
Magnar BjøråsDepartment of Microbiology, University of Oslo, and Oslo University Hospital, Rikshospitalet, Oslo, Norway. magnar.bjoras@ntnu.no.
James Alexander BoothDepartment of Microbiology, University of Oslo, and Oslo University Hospital, Rikshospitalet, Oslo, Norway. james.booth@ntnu.no.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacterial toxin-antitoxin (TA) systems are genetic loci where the antitoxin gene product helps to control the expression or activity of the toxin gene product. Type I TA systems typically produce hydrophobic peptides that often localize to the inner membrane of bacteria. These amphipathic peptides can then potentially affect ion flows across the inner membrane. Here, we show that several type I toxins from Escherichia coli can affect depolarization, whereas tisB exclusively controls the depolarization of the proton gradient. tisB has been linked to persister cell formation following treatment with the antibiotic ciprofloxacin and tisB-istR has been implicated in the control of proton depolarization following treatment with ofloxacin. These results suggest that tisB could initiate the formation of persister cells by fully dissipating the proton gradient and that most of the electrical gradient greatly limiting ATP production following antibiotic-induced DNA damage.

Indexed as

Anti-Bacterial AgentsBacterial ToxinsDNA DamageEscherichia coliEscherichia coli ProteinsProtonsAdenosine TriphosphateCiprofloxacinToxin-Antitoxin SystemsAdenosine TriphosphateAnti-Bacterial AgentsBacterial ToxinsCiprofloxacinEscherichia coli ProteinsProtonsAntibioticsDNA damageE. coliMembrane depolarizationTisBType I toxin antitoxin systems

Identifiers

PMID40229382
PMCPMC11997105

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.