Evidence map›Paper›PMID 40234677›Full record

ArticleCommunications medicine2025

Hypothermic machine perfusion prevents hyperacute graft loss in pig-to-primate kidney xenotransplantation after 5-hours of cold Ischemia.

Yu Hisadome, Daniel L Eisenson, WeiLi Chen, Alexander C Schulick, Adam Luo, Michelle R Santillan, Kelly Casella, Du Gu, Mitsuhiro Sekijima, Hisashi Sahara and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors.

Yu HisadomeDepartment of Surgery, Division of Transplantation, The Johns Hopkins School of Medicine, Baltimore, MD, USA.
Daniel L EisensonDepartment of Surgery, Division of Transplantation, The Johns Hopkins School of Medicine, Baltimore, MD, USA.ORCID http://orcid.org/0000-0003-3868-0403
WeiLi ChenDepartment of Surgery, Division of Transplantation, The Johns Hopkins School of Medicine, Baltimore, MD, USA.
Alexander C SchulickDepartment of Surgery, Division of Transplantation, The Johns Hopkins School of Medicine, Baltimore, MD, USA.
Adam LuoDepartment of Oncology, The Johns Hopkins School of Medicine, Baltimore, MD, USA.ORCID http://orcid.org/0000-0002-3421-6913
Michelle R SantillanDepartment of Surgery, Division of Transplantation, The Johns Hopkins School of Medicine, Baltimore, MD, USA.ORCID http://orcid.org/0009-0005-9942-6240
Kelly CasellaDepartment of Oncology, The Johns Hopkins School of Medicine, Baltimore, MD, USA.
Du GuDepartment of Surgery, Division of Transplantation, The Johns Hopkins School of Medicine, Baltimore, MD, USA.
Mitsuhiro SekijimaDivision of Experimental Large Animal Research, Life Science and Laboratory Animal Research Unit, Kagoshima University, Kagoshima, Japan.
Hisashi SaharaDivision of Experimental Large Animal Research, Life Science and Laboratory Animal Research Unit, Kagoshima University, Kagoshima, Japan.
Daniel WarrenDepartment of Surgery, Division of Transplantation, The Johns Hopkins School of Medicine, Baltimore, MD, USA.
Andrew CameronDepartment of Surgery, Division of Transplantation, The Johns Hopkins School of Medicine, Baltimore, MD, USA.
Hayato IwaseDepartment of Surgery, Division of Transplantation, The Johns Hopkins School of Medicine, Baltimore, MD, USA.
Eugene ShenderovDepartment of Oncology, The Johns Hopkins School of Medicine, Baltimore, MD, USA.
Kazuhiko YamadaDepartment of Surgery, Division of Transplantation, The Johns Hopkins School of Medicine, Baltimore, MD, USA. kyamada6@jhmi.edu.ORCID http://orcid.org/0000-0002-0510-5843

Funding

XENOGRAFT TOLERANCE THROUGH MIXED CHIMERISMP01AI045897 · NIAID · MASSACHUSETTS GENERAL HOSPITAL · PI Megan Sykes · 2000 to 2026
$71.9M
NIAID NIH HHS P01 AI045897U.S. Department of Health & Human Services | National Institutes of Health (NIH) P01AI045897
6 · The paper itself

Abstract

backgroundXenotransplantation (XTx) is a promising strategy to address the organ shortage. Clinical application will likely require off-site procurement from designated pathogen-free (DPF) facilities, introducing unavoidable cold ischemic time (CIT). The impact of CIT and organ preservation method on graft function in XTx remains unclear.

methodsWe evaluated eight cases of pig-to-baboon kidney xenotransplantation performed after five hours of CIT, comparing static cold storage (SCS) to hypothermic machine perfusion (HMP) preservation. Outcomes were assessed relative to six additional pig-to-baboon transplants performed with minimal CIT.

resultsAll grafts preserved with SCS experience hyperacute rejection within 90 min of reperfusion, even in recipients with low levels of preformed anti-pig antibodies. In contrast, all HMP-preserved grafts reperfuse without clinical evidence of injury and maintain function for more than 14 days. Grafts transplanted with minimal CIT show similarly favorable outcomes.

conclusionsPorcine kidneys are highly sensitive to ischemia-reperfusion injury after cold preservation across xenogeneic barriers. Routine SCS leads to early graft failure, while HMP mitigates ischemic injury and may enable successful clinical XTx despite prolonged CIT.

Identifiers

PMID40234677
PMCPMC12000405

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.