ArticleJournal of Taibah University Medical Sciences2025
Exploring HPV-linked head and neck cancer in Southern Punjab, Pakistan: Insights from HPV-16 phylogenetic analysis.
Article in Journal of Taibah University Medical Sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introduction/objectives: Head and neck region squamous cell carcinoma (HNSCC) is a heterogeneous disease that can be categorized into human papillomavirus (HPV)-positive (20 %) and HPV-negative (80 %) subtypes. However, the prevalence of HPV genotypes is not clear in Pakistan. This study investigated how common the HPV-16 genotype is in patients with HNSCC in the Southern Punjab region of Pakistan, and the specific molecular features of this genotype. Methods: For this cross-sectional study, 85 tissue samples were collected from diagnosed cases of HNSCC. Formalin-fixed paraffin-embedded tissue sections were used for genomic DNA extraction. The L1 region was amplified using GP5+ and GP6+ primers to detect HPV DNA. Real-time PCR was conducted to genotype high-risk HPV (HR-HPV). Whole genome sequencing was used for phylogenetic analysis of HPV-16 and to detect mutations/single nucleotide polymorphisms (SNPs). Results: Among the 85 samples, 7.1 % were positive for HPV, where 4.7 % were positive for HPV-16 and 2.4 % were positive for HPV-18. A significant association was found between HR-HPV positivity and histological grade ( Conclusion: The findings obtained in the present study demonstrate the low prevalence of HR-HPV associated HNSCC in Pakistan. Phylogenetic analysis showed that HPV-16 genome isolated and sequenced in this study had a distinct genetic structure and it also shared similarities with genomes reported from Thailand, the United States, India, China, and Europe.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.