Evidence map›Paper›PMID 40238183›Full record

ArticleBioinformatics (Oxford, England)2025

Colora: a Snakemake workflow for complete chromosome-scale de novo genome assembly.

Lia Obinu, Timothy Booth, Heleen De Weerd, Urmi Trivedi, Andrea Porceddu

Abstract read
In one paragraph

Article in Bioinformatics (Oxford, England), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Lia ObinuDepartment of Agricultural Sciences, University of Sassari, Viale Italia 39/a, Sassari, Sardinia, 07100, Italy.ORCID 0000-0002-3208-323X
Timothy BoothEdinburgh Genomics, The University of Edinburgh, Ashworth Laboratories, The King's Buildings, Charlotte Auerbach Rd, Edinburgh, Scotland, EH9 3FL, United Kingdom.ORCID 0000-0003-2470-9519
Heleen De WeerdEdinburgh Genomics, The University of Edinburgh, Ashworth Laboratories, The King's Buildings, Charlotte Auerbach Rd, Edinburgh, Scotland, EH9 3FL, United Kingdom.
Urmi TrivediEdinburgh Genomics, The University of Edinburgh, Ashworth Laboratories, The King's Buildings, Charlotte Auerbach Rd, Edinburgh, Scotland, EH9 3FL, United Kingdom.
Andrea PorcedduDepartment of Agricultural Sciences, University of Sassari, Viale Italia 39/a, Sassari, Sardinia, 07100, Italy.ORCID 0000-0001-7126-9048

Funding

European UnionUniversity of Sassari
6 · The paper itself

Abstract

motivationDe novo assembly creates reference genomes that underpin many modern biodiversity and conservation studies. Large numbers of new genomes are being assembled by labs around the world. To avoid duplication of efforts and variable data quality, we desire a best-practice assembly process, implemented as an automated portable workflow.

resultsHere, we present Colora, a Snakemake workflow that produces chromosome-scale de novo primary or phased genome assemblies complete with organelles using Pacific Biosciences HiFi, Hi-C, and optionally Oxford Nanopore Technologies reads as input. Colora is a user-friendly, versatile, and reproducible pipeline that is ready to use by researchers looking for an automated way to obtain high-quality de novo genome assemblies. AVAILABILITY AND IMPLEMENTATION: The source code of Colora is available on GitHub (https://github.com/LiaOb21/colora) and has been deposited in Zenodo under DOI https://doi.org/10.5281/zenodo.13321576. Colora is also available at the Snakemake Workflow Catalog (https://snakemake.github.io/snakemake-workflow-catalog/? usage=LiaOb21%2Fcolora).

Indexed as

ChromosomesGenomeGenomicsSoftwareHigh-Throughput Nucleotide SequencingSequence Analysis, DNAWorkflow

Identifiers

PMID40238183
PMCPMC12065627

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.