Evidence map›Paper›PMID 40246940›Full record

ArticleScientific reports2025

Genome-wide identification and characterization of Subtilisin-like Serine protease encoding genes in Vigna radiata L. Wilczek.

Poornima Singh, Brijesh Pandey, Shalini Purwar, Ramakrishnan Madhavan Nair, Aditya Pratap

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Poornima SinghDepartment of Biotechnology, School of Life Sciences, Mahatma Gandhi Central University, Motihari, 845 401, India.
Brijesh PandeyDepartment of Biotechnology, School of Life Sciences, Mahatma Gandhi Central University, Motihari, 845 401, India.
Shalini PurwarDepartment of Basic and Social Sciences, Banda University of Agriculture and Technology, Banda, 210 001, India. shalinipurwarbuat@gmail.com.
Ramakrishnan Madhavan NairWorld Vegetable Centre, ICRISAT Campus, Hyderabad, 502 324, Telangana, India.
Aditya PratapProject Coordination Unit, All India Coordinated Research Project on Kharif Pulses, ICAR-Indian Institute of Pulses Research, Kanpur, 208 024, India. Aditya.Pratap@icar.gov.in.

Funding

International Mungbean Improvement Network 2 IMIN2
6 · The paper itself

Abstract

Subtilisin-like serine proteases (SBTs) are serine proteolytic enzymes that play various roles in plant growth, function and stress responses. Vigna glabrescens, a wild relative of mungbean known to be a potential donor of photo- and thermoperiod insensitivity, was characterized for thermotolerance through reproductive biology and gene expression profiling. Whole-genome sequencing of this species has not yet been performed; hence, genome-wide analysis of this species has not been explored. In the present study, a systematic analysis of SBT-encoding genes in the V. radiata (Vradi_SBT) genome was conducted, with a focus on their response during flower development under different temperature regimes, such as optimum temperature, heat and cold stresses, in Vigna radiata and a wild relative, Vigna glabrescens. Thirty-eight Vradi_SBT genes were identified in the V. radiata genome and were further grouped into five distinct subgroups. The key domain of the SBT peptidase, "peptidase_S8_53," was found in all 38 Vradi_SBT proteins, while 28 of them contained the "peptidase_S8" domain. Additionally, 30 of these proteins contained a maximum of 10 motifs. A total of 22 orthologous genes were identified in Arabidopsis thaliana, whereas paralogous gene pairs were detected as tandemly duplicated genes with V. unguiculata. Cis-acting element analysis revealed that these genes presented more stress-responsive promoter sequences than the other promoters. Furthermore, Vradi_SBT-1.9 was found significantly upregulated under both high- and low-temperature stresses. This study provides insights into SBT-encoding genes and their possible role in flower development and thermotolerance in Vigna species.

Indexed as

Genome, PlantPlant ProteinsSerine ProteasesSubtilisinsVignaFlowersGene Expression ProfilingGene Expression Regulation, PlantPhylogenyStress, PhysiologicalPlant ProteinsSerine ProteasesSubtilisinsCold stressCWRHeat stressSubtilisin-like serine proteaseVigna

Identifiers

PMID40246940
PMCPMC12006535

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.