Evidence map›Paper›PMID 40249433›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Detection of Viral RNA in Paraffinized Tissues by Nested RT-PCR.

Yuli Souza, Pedro Paulo Manso, Marcelo Pelajo-Machado, Barbara de Oliveira

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yuli SouzaLaboratory of Modeling of Biological Systems (LAMES), Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, RJ, Brazil.
Pedro Paulo MansoLaboratory of Modeling of Biological Systems (LAMES), Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, RJ, Brazil.
Marcelo Pelajo-MachadoLaboratory of Modeling of Biological Systems (LAMES), Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, RJ, Brazil.
Barbara de OliveiraLaboratory of Modeling of Biological Systems (LAMES), Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, RJ, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tissue fixed by formalin and embedded in paraffin is widely used in research and histological routine due to its good morphological preservation. Detection of viral RNA finds in these materials a barrier to be overcome. The fixation by formaldehyde, high temperatures used in the processing of the material, and the embedding in paraffin usually degrade the RNA. Such procedures make it difficult to extract RNA and isolate in adequate quality to be detected by the RT-PCR technique. Here we show an efficient protocol for the detection of yellow fever virus RNA in formalin-fixed paraffin-embedded (FFPE) tissue samples using the Nested RT-PCR technique.

Indexed as

Paraffin EmbeddingReverse Transcriptase Polymerase Chain ReactionRNA, ViralYellow fever virusFormaldehydeHumansTissue FixationFormaldehydeRNA, ViralConventional PCRFFPE tissue samplesNested RT-PCRParaffinized tissueYellow fever genome detectionyellow fever RNA

Identifiers

PMID40249433

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.