Evidence mapPaperPMID 40249477Full record

ArticleOdontology2026

Effects of universal adhesives on dentin matrix proteins, matrix metalloproteinases and cytokine release of human pulp cells.

Bilge Ersöz, Numan Aydin, Elif Aybala Oktay, İrem Kübra Çal, Serpil Karaoğlanoğlu

Abstract read
In one paragraph

Article in Odontology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Bilge ErsözGulhane Faculty of Dentistry, Department of Restorative Dental Treatment, University of Health Sciences, 06018, Ankara, Turkey.ORCID http://orcid.org/0000-0003-0769-0457
Numan AydinGulhane Faculty of Dentistry, Department of Restorative Dental Treatment, University of Health Sciences, 06018, Ankara, Turkey.ORCID http://orcid.org/0000-0001-8628-4507
Elif Aybala OktayGulhane Faculty of Dentistry, Department of Restorative Dental Treatment, University of Health Sciences, 06018, Ankara, Turkey.ORCID http://orcid.org/0000-0003-1668-0592
İrem Kübra ÇalGulhane Faculty of Dentistry, Department of Restorative Dental Treatment, University of Health Sciences, 06018, Ankara, Turkey.ORCID http://orcid.org/0009-0008-1199-6989
Serpil KaraoğlanoğluGulhane Faculty of Dentistry, Department of Restorative Dental Treatment, University of Health Sciences, 06018, Ankara, Turkey. serpil.karaoglanoglu@sbu.edu.tr.ORCID http://orcid.org/0000-0003-0601-8028

Funding

Scientific Research Committee of the Health Sciences University 2022/163
6 · The paper itself

Abstract

The potential toxicity of universal adhesives, which contain various monomers, solvents and fillers is a significant research topic. This study aims to investigate the toxicity and effects of universal adhesives on dentin matrix proteins (DMP-1), matrix metalloproteinases (MMP-2, MMP-8), tissue inhibitors of metalloproteinase-1 (TIMP-1), and cytokines [tumor necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1)] in pulp cell lines. Six universal adhesives [Gluma Bond Universal (GBU), Prime&Bond Universal (PBU), Clearfil S3 Universal Bond (CS3UB), OptiBond Universal (OBU), G-2 Bond Universal (G2BU) and Bond Force II (BFII)] were assessed using MTT and ELISA cytotoxicity tests. The data obtained from MTT and ELISA tests were analyzed using two-way analysis of variance (ANOVA). The 1:1 extracts of BFII and GBU showed higher cell viability at 24 and 48 h compared to PBU, CS3B, OBU, and G2B adhesives (p < 0.001), and furthermore, the 1:1 extracts of GBU showed statistically the highest cell viability at 72 h (p < 0.001). The universal adhesives tested showed a significant decrease in TIMP-1 in pulp cells (p < 0.05), while TNF-α, IL-1, DMP-1, MMP-2 and MMP-8 levels did not change significantly. The tested adhesives exhibited varying degrees of cytotoxic effects depending on time and dose. The results indicate that the composition of these adhesives plays a crucial role in their cytotoxicity and impact on pulp cell viability. The amount and duration of adhesive application should be carefully regulated to maintain biocompatibility and ensure safe usage.

Indexed as

CytokinesDental CementsDental PulpDentin-Bonding AgentsExtracellular Matrix ProteinsMatrix MetalloproteinasesCell LineCell SurvivalEnzyme-Linked Immunosorbent AssayHumansMaterials TestingMatrix Metalloproteinase 2Resin CementsTissue Inhibitor of Metalloproteinase-1Tumor Necrosis Factor-alphaCytokinesDental CementsDentin-Bonding AgentsExtracellular Matrix ProteinsMatrix Metalloproteinase 2Matrix MetalloproteinasesResin CementsTissue Inhibitor of Metalloproteinase-1Tumor Necrosis Factor-alphaAdhesivesCytotoxicityDentin matrix protein 1Matrix metalloproteinasesPulp cells

Identifiers

PMID40249477
PMCPMC12795904

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.