Evidence map›Paper›PMID 40249481›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

In Vivo Two-Photon Imaging of the Olfactory System in Insects.

Marco Paoli, Albrecht Haase

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Marco PaoliNeuroscience Paris-Seine - Institut de Biologie Paris-Seine, Sorbonne Université, INSERM, CNRS, Paris, France. marco.paoli@sorbonne-universite.fr.
Albrecht HaaseCenter for Mind/Brain Sciences and Department of Physics, University of Trento, Trento, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter describes how to apply two-photon neuroimaging to study the insect olfactory system in vivo. It provides a complete protocol for insect brain functional imaging, with additional remarks on the acquisition of morphological information from the living brain. We discuss the most important choices to make when buying or building a two-photon laser scanning microscope. We illustrate different possibilities of animal preparation and brain tissue labeling for in vivo imaging. Finally, we give an overview of the main methods of image data processing and analysis, together with practical examples of pioneering applications of this imaging modality.

Indexed as

InsectaMicroscopy, Fluorescence, MultiphotonOlfactory PathwaysAnimalsBrainImage Processing, Computer-AssistedMicroscopy, ConfocalAntennal lobeCalcium imagingDrosophilaHoney beeInsectsOlfactionTwo-photon microscopy

Identifiers

PMID40249481

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.