Evidence map›Paper›PMID 40249709›Full record

ArticleSTAR protocols2025

Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig.

Yasmin Eckstein, Barbara Kessler, Arne Hinrichs, Istvan Novak, Anne von Thaden, Timo Lorenzen, Birgit Rathkolb, Armin Scholz, Andreas Blutke, Sietse-Jan Koopmans and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Yasmin EcksteinChair for Molecular Animal Breeding and Biotechnology, Gene Center and Department of Veterinary Sciences, LMU Munich, 81377 Munich, Germany; Center for Innovative Medical Models (CiMM), LMU Munich, 85764 Oberschleißheim, Germany.
Barbara KesslerChair for Molecular Animal Breeding and Biotechnology, Gene Center and Department of Veterinary Sciences, LMU Munich, 81377 Munich, Germany; Center for Innovative Medical Models (CiMM), LMU Munich, 85764 Oberschleißheim, Germany.
Arne HinrichsChair for Molecular Animal Breeding and Biotechnology, Gene Center and Department of Veterinary Sciences, LMU Munich, 81377 Munich, Germany; Center for Innovative Medical Models (CiMM), LMU Munich, 85764 Oberschleißheim, Germany.
Istvan NovakChair for Molecular Animal Breeding and Biotechnology, Gene Center and Department of Veterinary Sciences, LMU Munich, 81377 Munich, Germany; Center for Innovative Medical Models (CiMM), LMU Munich, 85764 Oberschleißheim, Germany.
Anne von ThadenGerman Center for Neurodegenerative Diseases (DZNE), LMU Munich, 81377 Munich, Germany.
Timo LorenzenInstitute of Veterinary Pathology at the Center for Clinical Veterinary Medicine, LMU Munich, 80539 Munich, Germany.
Birgit RathkolbChair for Molecular Animal Breeding and Biotechnology, Gene Center and Department of Veterinary Sciences, LMU Munich, 81377 Munich, Germany; German Mouse Clinic (GMC) and Genome Analysis Center (GAC), Institute of Experimental Genetics, Helmholtz Munich, 85764 Neuherberg, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, Germany.
Armin ScholzLivestock Center Oberschleissheim, LMU Munich, 85764 Oberschleißheim, Germany.
Andreas BlutkeInstitute of Veterinary Pathology at the Center for Clinical Veterinary Medicine, LMU Munich, 80539 Munich, Germany.
Sietse-Jan KoopmansWageningen Livestock Research, Wageningen University & Research, Wageningen, the Netherlands.
Martin Hrabĕ de AngelisGerman Mouse Clinic (GMC) and Genome Analysis Center (GAC), Institute of Experimental Genetics, Helmholtz Munich, 85764 Neuherberg, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, Germany; Chair of Experimental Genetics, TUM School of Life Sciences (SoLS), Technische Universität München, 85354 Freising, Germany.
Berit Østergaard ChristoffersenNovo Nordisk A/S, Maaloev, Denmark.
Eckhard WolfChair for Molecular Animal Breeding and Biotechnology, Gene Center and Department of Veterinary Sciences, LMU Munich, 81377 Munich, Germany; Center for Innovative Medical Models (CiMM), LMU Munich, 85764 Oberschleißheim, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, Germany; Interfaculty Center for Endocrine and Cardiovascular Disease Network Modelling and Clinical Transfer (ICONLMU), LMU Munich, 81377 Munich, Germany.
Simone RennerChair for Molecular Animal Breeding and Biotechnology, Gene Center and Department of Veterinary Sciences, LMU Munich, 81377 Munich, Germany; Center for Innovative Medical Models (CiMM), LMU Munich, 85764 Oberschleißheim, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, Germany; Interfaculty Center for Endocrine and Cardiovascular Disease Network Modelling and Clinical Transfer (ICONLMU), LMU Munich, 81377 Munich, Germany. Electronic address: simone.renner@lmu.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter implantation, testing of intravenous glucose tolerance, performance of hyperinsulinemic-euglycemic clamps (HECs) and hyperglycemic clamps (HGCs), and blood processing. We then detail procedures for analysis of plasma glucose, insulin, glucagon, and C-peptide concentrations as well as data analysis. For complete details on the use and execution of this protocol, please refer to Renner et al.

Indexed as

Blood GlucoseGlucose Clamp TechniqueInsulinInsulin SecretionAnimalsC-PeptideGlucagonGlucose Tolerance TestSwineBlood GlucoseC-PeptideGlucagonInsulinBiotechnology and bioengineeringMetabolismModel Organisms

Identifiers

PMID40249709
PMCPMC12224880

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.