ArticleInternational journal of ophthalmology2025
Inhibition of IGF-1Rα affects the differentiation fate of rat optic cup-derived retinal stem cells to retinal ganglion cells
Article in International journal of ophthalmology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
aimTo explore the impact of insulin-like growth factor-1 receptor α (IGF-1Rα) on the differentiation fate of optic-cup-derived retinal stem cells (OC-RSCs) into retinal ganglion cells (RGCs)
methodsOC-RSCs were isolated from optic cups of rats on embryonic day 12.5, and high-purity OC-RSCs were obtained by conditioned culture and passage. Differentiation of OC-RSCs into RGCs under different serum concentrations was examined using flow cytometry, and the serum concentration with high interference with differentiation ratio was selected. Furthermore, the effect of blocking IGF-1Rα on the differentiation of OC-RSCs into RGCs was analyzed through immunocytochemistry and Western blotting.
resultsImmunohistochemical analysis revealed IGF-1Rα was highly expressed in rat embryos at day 12.5. OC-RSCs were isolated and purified, and high-purity OC-RSCs were obtained. When 2.5% serum was administered, the ratio of differentiated RGCs (Thy-1.1 positive) decreased significantly, and the results of immunoblotting also confirmed the blockade of IGF-1Rα reduced Thy-1.1 protein expression.
conclusionIGF-1Rα blocking can reduce the differentiation of OC-RSCs into RGCs.
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