Evidence map›Paper›PMID 40260404›Full record

ArticleFrontiers in molecular biosciences2025

Influence of expression and purification protocols on Gα biochemical activity: kinetics of plant and mammalian G protein cycles.

Timothy E Gookin, David Chakravorty, Sarah M Assmann

Abstract read
In one paragraph

Article in Frontiers in molecular biosciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Is GCR1 the GPR157 of plants?Plant physiology · 2025
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

3 authors.

Timothy E Gookin *Biology Department, Pennsylvania State University, University Park, PA, United States.
David Chakravorty *Biology Department, Pennsylvania State University, University Park, PA, United States.
Sarah M AssmannBiology Department, Pennsylvania State University, University Park, PA, United States.

Funding

Regulation of heterotrimeric G protein signaling by subunit phosphorylationR01GM126079 · NIGMS · PENNSYLVANIA STATE UNIVERSITY, THE · PI ASSMANN, SARAH M · 2018 to 2022
$1.5M
Phospho- and natural-variant regulation of heterotrimeric G protein signalingR35GM153492 · NIGMS · PENNSYLVANIA STATE UNIVERSITY, THE · PI Sarah M Assmann · 2024 to 2026
$1.2M
NIGMS NIH HHS R01 GM126079NIGMS NIH HHS R35 GM153492
6 · The paper itself

Abstract

Heterotrimeric G proteins, composed of Gα, Gβ, and Gγ subunits, are a class of signal transduction complexes with broad roles in human health and agriculturally relevant plant physiological and developmental traits. In the classic paradigm, guanine nucleotide binding to the Gα subunit regulates the activation status of the complex. We sought to develop improved methods for heterologous expression and rapid purification of Gα subunits, initially targeting GPA1, the sole canonical Gα subunit of the model plant species,

Indexed as

BODIPYGNAI1GNAO1GPA1GTPheterotrimeric G proteinrecombinant protein expressionSYPRO orange

Identifiers

PMID40260404
PMCPMC12009698

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.