Evidence mapPaperPMID 40263272Full record

ArticleNature communications2025

Acoustic loudness factor as an experimental parameter for benchmarking small molecule photoacoustic probes.

Frederik Brøndsted, Julia L McAfee, Jerimiah D Moore, Harry R Shield, Luca Menozzi, Xinqi Zhou, Yuan Fang, Ruwen Yin, Junjie Yao, Kelsey P Kubelick and 1 more

Abstract read
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Frederik Brøndsted *Department of Chemistry, University of Virginia, Charlottesville, VA, 22904, USA.
Julia L McAfee *Department of Chemistry, University of Virginia, Charlottesville, VA, 22904, USA.
Jerimiah D MooreDepartment of Chemistry, University of Virginia, Charlottesville, VA, 22904, USA.
Harry R ShieldDepartment of Chemistry, University of Virginia, Charlottesville, VA, 22904, USA.
Luca MenozziDepartment of Biomedical Engineering, Duke University, Durham, NC, 27708, USA.ORCID http://orcid.org/0000-0001-7542-1051
Xinqi ZhouDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
Yuan FangDepartment of Chemistry, University of Virginia, Charlottesville, VA, 22904, USA.ORCID http://orcid.org/0000-0003-1987-0885
Ruwen YinDepartment of Chemistry, University of Virginia, Charlottesville, VA, 22904, USA.
Junjie YaoDepartment of Biomedical Engineering, Duke University, Durham, NC, 27708, USA.ORCID http://orcid.org/0000-0002-2381-706X
Kelsey P KubelickDepartment of Biomedical Engineering, University of Virginia, Charlottesville, VA, 22908, USA.ORCID http://orcid.org/0000-0002-6342-2994
Cliff I StainsDepartment of Chemistry, University of Virginia, Charlottesville, VA, 22904, USA. cstains@virginia.edu.ORCID http://orcid.org/0000-0002-3165-4539

Funding

High-throughput Imaging-integrated Vascular Model for Understanding Thromboembolism and Therapeutics ScreeningR01HL166522 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · 2024 to 2025
$1.3M
Chemical Approaches for Interrogating Fundamental Biomedical ProcessesR35GM148221 · NIGMS · UNIVERSITY OF VIRGINIA · 2023 to 2025
$781k
3D real-time super-resolution cavitation mapping in laser lithotripsy of urinary stone diseaseR01DK139109 · DUKE UNIVERSITY · 2025 to 2025
$602k
National Science Foundation (NSF) 2144788National Science Foundation (NSF) DGE-2139754NHLBI NIH HHS R01 HL166522NIDDK NIH HHS R01 DK139109NIGMS NIH HHS R01 GM134036NIGMS NIH HHS R35 GM148221NIH HHS S10 OD030409NINDS NIH HHS R01 NS111039U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (NHLBI) R01HL166522U.S. Department of Health & Human Services | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (National Institute of Diabetes & Digestive & Kidney Diseases) R01DK139109U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) R01GM134036U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) R35GM148221U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) R01NS111039U.S. Department of Health & Human Services | NIH | NIH Office of the Director (OD) OD030409
6 · The paper itself

Abstract

Photoacoustic imaging (PAI) is an emerging biomedical imaging modality with promise as a point-of-care diagnostic. This imaging modality relies on optical excitation of an absorber followed by production of ultrasound through the photoacoustic effect, resulting in high spatial resolution with imaging depths in the centimeter range. Herein, we disclose the discovery of the first benchmarking parameter for small molecule dye performance in PAI, which we term the acoustic loudness factor (ALF). ALF can predict dye performance in PAI without the need for access to photoacoustic instrumentation and can be used to guide the systematic evaluation of design strategies to enhance photoacoustic signal. Lastly, we demonstrate that enhancements in ALF can be translated to in vivo PAI. Akin to the use of fluorescence brightness in fluorophore design and evaluation for fluorescence imaging, we anticipate that ALF will guide the design and evaluation of improved probes for PAI.

Indexed as

AcousticsPhotoacoustic TechniquesAnimalsBenchmarkingFluorescent DyesHumansMiceOptical ImagingFluorescent Dyes

Identifiers

PMID40263272
PMCPMC12015456

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.