Evidence map›Paper›PMID 40269178›Full record

ArticleScientific reports2025

Gene regulatory networks analysis for the discovery of prognostic genes in gliomas.

Pedro Marçal Barcelos, Igor Salerno Filgueiras, Adriel Leal Nóbile, Júlia Nakanishi Usuda, Anny Silva Adri, Débora Gomes de Alburquerque, Yohan Lucas Gonçalves Côrrea, Fernando Yuri Nery do Vale, Ian Antunes Ferreira Bahia, Roseane Galdioli Nava and 9 more

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Pedro Marçal Barcelos *Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo (USP), Av. Prof. Lineu Prestes, 580, São Paulo, SP, 05508-000, Brazil. pedrombarcelos73@gmail.com.
Igor Salerno FilgueirasDepartment of Immunology, Institute of Biomedical Sciences, University of São Paulo (USP), São Paulo, SP, Brazil.
Adriel Leal NóbileDepartment of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo (USP), Av. Prof. Lineu Prestes, 580, São Paulo, SP, 05508-000, Brazil.
Júlia Nakanishi UsudaDepartment of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo (USP), Av. Prof. Lineu Prestes, 580, São Paulo, SP, 05508-000, Brazil.
Anny Silva AdriDepartment of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo (USP), Av. Prof. Lineu Prestes, 580, São Paulo, SP, 05508-000, Brazil.
Débora Gomes de AlburquerqueLaboratory of Psychoneuroimmunology, Selye Lab, University of São Paulo School of Medicine, São Paulo, Brazil.
Yohan Lucas Gonçalves CôrreaDepartment of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo (USP), Av. Prof. Lineu Prestes, 580, São Paulo, SP, 05508-000, Brazil.
Fernando Yuri Nery do ValeDepartment of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo (USP), Av. Prof. Lineu Prestes, 580, São Paulo, SP, 05508-000, Brazil.
Ian Antunes Ferreira BahiaInterunit Postgraduate Program on Bioinformatics, Institute of Chemistry (IQ), University of São Paulo (USP), Matão Street, 1010, São Paulo, SP, 05508-090, Brazil.
Roseane Galdioli NavaLaboratory of Psychoneuroimmunology, Selye Lab, University of São Paulo School of Medicine, São Paulo, Brazil.
Mariana BoroniLaboratory of Bioinformatics and Computational Biology, Division of Experimental and Translational Research, Brazilian National Cancer Institute (INCA), Rio de Janeiro, RJ, Brazil.
Alexandre H C MarquesBioinformatics Multidisciplinary Environment, Federal University of Rio Grande do Norte, Natal, Brazil.
Rodrigo DalmolinBioinformatics Multidisciplinary Environment, Federal University of Rio Grande do Norte, Natal, Brazil.
Lena F SchimkeDepartment of Immunology, Institute of Biomedical Sciences, University of São Paulo (USP), São Paulo, SP, Brazil.
Gustavo Cabral-MirandaDepartment of Immunology, Institute of Biomedical Sciences, University of São Paulo (USP), São Paulo, SP, Brazil.
Helder I NakayaHospital Israelita Albert Einstein, São Paulo, SP, Brazil.
Haroldo Dutra DiasLaboratory of Psychoneuroimmunology, Selye Lab, University of São Paulo School of Medicine, São Paulo, Brazil.
Dennyson Leandro M Fonseca *Interunit Postgraduate Program on Bioinformatics, Institute of Chemistry (IQ), University of São Paulo (USP), Matão Street, 1010, São Paulo, SP, 05508-090, Brazil. dennyson@usp.br.
Otavio Cabral-Marques *Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo (USP), Av. Prof. Lineu Prestes, 580, São Paulo, SP, 05508-000, Brazil. otavio.cmarques@usp.br.

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico 309482/2022-4Conselho Nacional de Desenvolvimento Científico e Tecnológico 310376/2022-0Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 88887.699840/2022-00Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 88887.801068/2023-00Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro E-26/201.322/2022 (272260)Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro E-26/205.530/2022 (284921)Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro E-26/210.302/2022 (270388)Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro E-26/211.648/2021 (269502)Fundação de Amparo à Pesquisa do Estado de São Paulo 2018/18886-9Fundação de Amparo à Pesquisa do Estado de São Paulo 2020/01688-0Fundação de Amparo à Pesquisa do Estado de São Paulo 2020/07069-0Fundação de Amparo à Pesquisa do Estado de São Paulo 2020/16246-2Fundação de Amparo à Pesquisa do Estado de São Paulo 2023/06086-6Fundação de Amparo à Pesquisa do Estado de São Paulo 2023/07806-2Fundação de Amparo à Pesquisa do Estado de São Paulo 2023/12268-0Fundação de Amparo à Pesquisa do Estado de São Paulo 2023/13356-0Fundação de Amparo à Pesquisa do Estado de São Paulo 2023/14417-2
6 · The paper itself

Abstract

Gliomas are the most common and aggressive primary tumors of the central nervous system. Dysregulated transcription factors (TFs) and genes have been implicated in glioma progression, yet these tumors' overall structure of gene regulatory networks (GRNs) remains undefined. We analyzed transcriptional data from 989 primary gliomas in The Cancer Genome Atlas (TCGA) and the Chinese Glioma Genome Atlas (CGGA) to address this. GRNs were reconstructed using the RTN package which identifies regulons-sets of genes regulated by a common TF based on co-expression and mutual information. Regulon activity was evaluated through Gene Set Enrichment Analysis. Elastic net regularization and Cox regression identified 31 and 32 prognostic genes in the TCGA and CGGA datasets, respectively, with 11 genes overlapping, many of which are associated with neural development and synaptic processes. GAS2L3, HOXD13, and OTP demonstrated the strongest correlations with survival outcomes among these. Single-cell RNA-seq analysis of 201,986 cells revealed distinct expression patterns for these genes in glioma subpopulations, particularly oligoprogenitor cells. This study uncovers key GRNs and prognostic genes in gliomas, offering new insights into tumor biology and potential therapeutic targets.

Indexed as

Biomarkers, TumorBrain NeoplasmsGene Expression Regulation, NeoplasticGene Regulatory NetworksGliomaGene Expression ProfilingHumansPrognosisTranscription FactorsBiomarkers, TumorTranscription FactorsGliomaRegulonsSurvival

Identifiers

PMID40269178
PMCPMC12018930

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.