Evidence map›Paper›PMID 40269882›Full record

ArticleDiagnostic pathology2025

High B7-H3 protein expression in Medulloblastoma is associated with metastasis and unfavorable patient outcomes.

Patrícia Fontão, Gustavo Ramos Teixeira, Daniel Antunes Moreno, Rui Ferreira Marques, João Norberto Stavale, Suzana Maria Fleury Malheiros, Carlos Almeida Júnior, Bruna Minniti Mançano, Rui Manuel Reis

Abstract read
In one paragraph

Article in Diagnostic pathology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. B7-H3 Gene Expression Shapes Prognosis and Therapeutic Opportunities across Patient Groups with Prostate Cancer.Clinical cancer research : an official journal of the American Association for Cancer Research · 2026
    Article
  4. Review
  5. Article
  6. Review
  7. Review
  8. Review
  9. Article
  10. Review
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Patrícia FontãoMolecular Oncology Research Center, Barretos Cancer Hospital, Rua Antenor Duarte Vilela, 1331, Barretos, SP, 14784-400, Brazil.
Gustavo Ramos TeixeiraBarretos School of Health Sciences, Dr. Paulo Prata - FACISB, Barretos, São Paulo, Brazil.
Daniel Antunes MorenoMolecular Oncology Research Center, Barretos Cancer Hospital, Rua Antenor Duarte Vilela, 1331, Barretos, SP, 14784-400, Brazil.
Rui Ferreira MarquesLife and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal.
João Norberto StavaleFederal University of São Paulo, São Paulo, Brazil.
Suzana Maria Fleury MalheirosFederal University of São Paulo, São Paulo, Brazil.
Carlos Almeida JúniorBarretos Children's Cancer Hospital, Barretos, Brazil.
Bruna Minniti MançanoMolecular Oncology Research Center, Barretos Cancer Hospital, Rua Antenor Duarte Vilela, 1331, Barretos, SP, 14784-400, Brazil.
Rui Manuel ReisMolecular Oncology Research Center, Barretos Cancer Hospital, Rua Antenor Duarte Vilela, 1331, Barretos, SP, 14784-400, Brazil. ruireis.hcb@gmail.com.

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico Productivity fellowshipFundação para a Ciência e a Tecnologia 2021.07647.BDMS-SECTICS-Decit/CNPq DTI-A MS-SECTICS-Decit/CNPq n° 16/2023
6 · The paper itself

Abstract

backgroundMedulloblastoma (MB) is the most common malignant brain tumor in children. Although the 5-year survival rate is approximately 70-80%, the current standard treatment results in severe and long-term side effects. The search for new anticancer immunotherapeutic targets has identified B7-H3 as a promising candidate in various solid tumors. However, the role of B7-H3 in MB remains unclear, and studies reporting its protein expression and association with clinicopathological characteristics are still limited.

methodsIn this study, B7-H3 protein expression was evaluated by immunohistochemistry in seven non-tumor samples and 43 molecularly characterized MB tissues. Its expression profile was correlated with B7-H3 (CD276) mRNA levels, which were previously determined by nCounter, as well as with the patients' clinical features.

resultsOnly 14.3% (1/7) of non-tumor brain and cerebellum tissues showed B7-H3 positivity, whereas 95.6% (41/43) of the MB samples expressed this protein at distinct levels. B7-H3 was found in the cytoplasm and on the membrane of cancer cells. A significant positive correlation was observed between CD276 mRNA and B7-H3 protein levels. Moreover, high expression of B7-H3 protein was associated with worse overall survival and the presence of metastasis at diagnosis.

conclusionsThis is the first study to associate CD276 mRNA and B7-H3 protein levels in MB, revealing a significant positive correlation. We observed that B7-H3 was overexpressed in MB compared to non-tumor brain tissue. High B7-H3 expression was associated with a worse outcome and with the presence of metastasis at diagnosis.

Indexed as

B7 AntigensBiomarkers, TumorCerebellar NeoplasmsMedulloblastomaAdolescentChildChild, PreschoolFemaleHumansImmunohistochemistryInfantMalePrognosisB7 AntigensBiomarkers, TumorCD276 protein, humanB7-H3CD276ImmunotherapyMedulloblastomaMetastasisPrognostic marker

Identifiers

PMID40269882
PMCPMC12016131

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.