ArticleChemistry (Weinheim an der Bergstrasse, Germany)2025
Click-Functionalized Cyanine Fluorogenic Dimers for Improved Detection of GPCRs: Application to Imaging of ApelinR in Living Cells.
Article in Chemistry (Weinheim an der Bergstrasse, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Decoupling Photophysical Brightness and Cellular Imaging Efficiency in 4-ANI-Based Fluorophores: The Critical Role of Sterically Hindered N-Substituents.Chembiochem : a European journal of chemical biology · 2026Article
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11 authors.
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Abstract
Fluorogenic dimers enable background-free imaging of biological targets under wash-free conditions owing to a strong fluorescence enhancement in the apolar cell microenvironment. However, it is crucial that the imaging probe interacts solely with the target receptor to avoid nonspecific interactions and ensure detection with a high signal-to-noise ratio. Herein, we describe a convenient and rapid approach for the synthesis of various functionalized cyanine dyes by click chemistry allowing the fine-tuning of the physicochemical and fluorogenic properties of the dimers. A structure-interaction relationship study was conducted for the fluorogenic dimers in the presence of bovine serum albumin (BSA) and liposomes as models of serum proteins and cell membranes. We identified d─Cy─E which combined the lowest nonspecific interactions with the optimal fluorescence turn-on properties. By conjugating d─Cy─E to a peptide ligand of the apelin GPCR, we developed Ap─d─Cy─E, the first fluorescent turn-on probe for the background-free imaging of this receptor in living cells.
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