ArticleProtoplasma2025
Establishing a reliable protoplast system for grapevine: isolation, transformation, and callus induction.
Article in Protoplasma, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Metabolomic and volatile profiling reveals defence-related effects of IbPep1 in sweet potato cell culture.BMC plant biology · 2026Article
- A novel protocol for protoplast isolation, transfection, and culture in Cannabis sativa L.BMC plant biology · 2025Article
- PVP-40 mediated enhancement of mesophyll protoplast yield and viability for transient gene expression in black huckleberry.Plant methods · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Protoplasts are single cells enclosed by the plasma membrane after cell wall removal. They are widely used in various biotechnological applications, including gene functional analysis, verification of genome editing reagents, and plant regeneration. Recent advances in genome editing have enabled the production of non-chimeric and transgene-free genome-edited plants using protoplasts. This process involves protoplast isolation, transformation, and regeneration, requiring advanced technical skills. Challenges in isolation and regeneration have limited their use in genome editing. In grapevines, however, very few studies have reported the use of protoplasts isolated from leaves. Efficient isolation and transformation protocols for Chardonnay remain lacking and require cultivar-specific optimization. In this study, we established a reliable and efficient protoplast isolation and transformation system by optimizing conditions for protoplast isolation and PEG-mediated transformation in Chardonnay cultivar. The yield of viable protoplasts was approximately 75 × 10
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What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.